Novel predictor discovery of plaque rapture in pathological mechanism of atherosclerosis development

• Project/Area Number: 26861112
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Kumamoto University
• Principal Investigator: Tian Zhe 熊本大学, 大学院生命科学研究部 (医), 研究員 (80723890)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 動脈硬化

Outline of Final Research Achievements

We performed the analysis of Angptl2 mRNA expression level in aorta of ApoE knockout mice with chronic administration of nicotin using osmotic pumps. The mRNA expression level of Angptl2 in aorta at 6 week after nicotin administration was significantly increased compared with vehicle control. Moreover, the mRNA expression level of Angptl2 in mouse primary endothelial cells with nicotin treatment was also significantly increased compared with control. To investigate whether the development of atherosclerotic plaque with nicotin treatment was dependent on Angptl2, we analyzed the atherosclerotic plaque of Angptl2; ApoE double KO (DKO) mice with 6-week nicotin administration. No differences were observed in size of atherosclerotic plaques of DKO mice between nicotin administration and vehicle group. These data suggested that nicotin induced Angptl2 expression in endothelial cells and then may promote atherosclerotic plaque.

Research Products

• [Journal Article] Angiopoietin-like protein 2 increases renal fibrosis by accelerating transforming growth factor-β signaling in chronic kidney disease. (2016)
  • Author(s): Jun Morinaga, Tsuyoshi Kadomatsu, Keishi Miyata, Motoyoshi Endo, Kazutoyo Terada, Zhe Tian, Taichi Sugizaki, Hiroki Tanigawa, Jiabin Zhao, Shunshun Zhu, Michio Sato, Kimi Araki, Ken-ichi Iyama, Kengo Tomita, Masashi Mukoyama, Kimio Tomita, Kenichiro Kitamura, Yuichi Oike
  • Journal Title: Kidney International Volume: 89 Issue: 2 Pages: 327-341
  • DOI: 10.1016/j.kint.2015.12.021
  • Peer Reviewed
• [Journal Article] Targeting complement anaphylatoxin C5a receptor in hyperoxic lung injury in mice. (2014)
  • Author(s): Xu Y, Tian Z, Xie P.
  • Journal Title: Mol Med Rep. Volume: 10(4) Issue: 4 Pages: 1786-92
  • DOI: 10.3892/mmr.2014.2394
  • Peer Reviewed
• [Journal Article] SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway. (2014)
  • Author(s): Yoshizawa T, Karim Md. F, Sato Y, Senokuchi T, Miyata K, Fukuda T, Go C, Tasaki M, Uchimura K, Kadomatsu T, Tian Z, Smolka C, Sawa T, Kakeya M, Tomizawa K, Ando Y, Araki E, Akaike T, Braun T, Oike Y, Bober E, Yamagata K.
  • Journal Title: Cell Metab. Volume: 9 Issue: 4 Pages: 712-721
  • DOI: 10.1016/j.cmet.2014.03.006
  • Peer Reviewed / Open Access