Statin ameliorates lipopolysaccharide-induced dysfunction of blood-brain barrier
Project/Area Number |
26861156
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurosurgery
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Research Institution | Nagasaki University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 血液脳関門 / スタチン / 脳虚血 / 中枢神経系疾患 / 炎症 |
Outline of Final Research Achievements |
Statins have a neuroprotective effect in neurological diseases, a pleiotropic effect possibly related to blood-brain barrier (BBB) function. In this study, we investigated the effects of statin on lipopolysaccharide (LPS)-induced BBB dysfunction in in vitro BBB model comprising co-culture of primary mouse brain endothelial cells, pericytes, and astrocytes. LPS (1 ng/ml, 24 hr) induced the impairment of permeability and transendothelial electrical resistance (TEER). Co-administration of statin alleviated the deleterious effects of LPS on BBB permeability and TEER. LPS increased IL-6, G-CSF, KC, MCP-1, RANTES release from our BBB model. Statin inhibited all these LPS-induced cytokine production. These data suggest that pitavastatin could ameliorate LPS-induced BBB dysfunction, which might be mediated by inhibiting LPS-induced cytokine production. Our present finding sheds light on the pharmacological significance of statins in various kinds of CNS diseases.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] A Stroke Code and cooperation between doctors and nurses could reduce door-to-needle time for acute ischemic stroke2018
Author(s)
Tateishi Y, Hamabe J, Kanamoto T, Nakaoka K, Yoshimura S, Morofuji Y, Horie N, Izumo T, Shiraishi H, Tsujino A.
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Journal Title
Japanese Journal of Stroke
Volume: 40
Issue: 1
Pages: 11-18
DOI
NAID
ISSN
0912-0726, 1883-1923
Related Report
Peer Reviewed
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[Journal Article] Lipopolysaccharide-induced blood-brain barrier disruption: roles of cyclooxygenase, oxidative stress, neuroinflammation, and elements of the neurovascular unit2015
Author(s)
Banks, W. A.; Gray, A. M.; Erickson, M. A.; Salameh, T. S.; Damodarasamy, M.; Sheibani, N.; Meabon, J. S.; Wing, E. E.; Morofuji, Y.; Cook, D. G.; Reed, M. J.
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Journal Title
J Neuroinflammation
Volume: 12
Issue: 1
Pages: 223-223
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Computational and in vitro studies of blast-induced blood-brain barrier disruption2015
Author(s)
Fukuda, S.; Nakagawa, S.; Tatsumi, R.; Morofuji, Y.; Takeshita, T.; Hayashi, K.; Tanaka, K.; Matsuo, T.; Niwa, M., Glucagon-Like Peptide-1 Strengthens the Barrier Integrity in Primary Cultures of Rat Brain Endothelial Cells Under Basal and Hyperglycemia Conditions. J Mol Neurosci 2015;
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Journal Title
ArXiv:1503.09157
Volume: -
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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