New approach for glioblastoma with immuno-chemotherapy using severe immunodeficiency mouse

• Project/Area Number: 26861159
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Nara Medical University
• Principal Investigator: MATSUDA RYOSUKE 奈良県立医科大学, 医学部, 助教 (60453164)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 脳腫瘍 / 膠芽腫 / NK細胞 / γδT細胞 / temozolomide / 免疫療法 / bisphosphonate / NOGマウス / Bisphosphonate / テモゾロミド / ベバシズマブ

Outline of Final Research Achievements

Our aim to investigate is to establish the new approach for glioblastoma. We demonstrated that combination therapy with gamma delta T cell and bisphosphonate had a significant strong anti-tumor effect for transplanted glioblastoma cells into NOG mouse. In second experiment, we demonstrated that NK cells had a strong anti-tumor effect for temozolomide-resistant glioblastoma cells in vitro. In conclusion, gamma delta T cell and NK cells are promising cell source for glioblastoma.

Research Products

• [Presentation] 膠芽腫細胞株におけるミノドロン酸の抗腫瘍効果の検討. (2015)
  • Author(s): 中村光利, 中澤務, 松田良介, 朴永銖, 本山靖, 弘中康雄, 中川一郎, 西村文彦, 丸谷明子, 尾本幸治, 田中祥貴, 中瀬裕之
  • Organizer: 第33回日本脳腫瘍学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 2015-12-07
• [Presentation] 膠芽腫細胞株におけるミノドロン酸の抗腫瘍効果の検討 (2015)
  • Author(s): 丸谷明子, 中村光利, 松田良介, 西村文彦, 朴永銖, 本山靖, 弘中康雄, 中川一郎, 田村健太郎, 竹島靖浩 , 尾本幸治, 田中祥貴, 中瀬裕之
  • Organizer: 第74回日本脳神経外科学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-10-14
• [Presentation] Minodronate exerts antitumor effects against human glioblastoma in cooperation with peripheral blood-derived γδ T cells (2015)
  • Author(s): Tsutomu Nakazawa, Mitsutoshi Nakamura, Ryosuke Matsuda, Young Soo Park, Yasushi Motoyama, Yasuo Hironaka, Fumihiko Nishimura, Ichiro Nakagawa, Kentaro Tamura, Yasuhiro Takeshima, Akiko Marutani, Kouji Omoto, Yoshitaka Tanaka, Yukiteru Ouji, Masahide Yoshikawa, Takahiro Tsujimura, Hiroyuki Nakase
  • Organizer: International Conference of Cancer Immunotherapy and Macrophages 2015
  • Place of Presentation: 東京
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] 膠芽腫細胞株におけるミノドロン酸の抗腫瘍効果の検討 (2015)
  • Author(s): 松田良介、中村光利、中澤務、西村文彦、辻村貴弘、中瀬裕之
  • Organizer: 日本免疫治療学研究会学術集会
  • Place of Presentation: 東京ガーデンパレス（東京都文京区）
  • Year and Date: 2015-02-28
• [Presentation] ヒト末梢血由来細胞傷害性γδT細胞は膠芽腫細胞株を殺傷する (2014)
  • Author(s): 中澤務、中村光利、松田良介、朴永銖、本山靖、新靖史、弘中康雄、中川一郎、西村文彦、丸谷明子、王寺典子、辻村貴弘、王寺幸輝、吉川正英、中瀬裕之
  • Organizer: 日本がん免疫学会学術集会
  • Place of Presentation: ひめぎんホール（愛媛県松山市道後町）
  • Year and Date: 2014-07-30 – 2014-08-01