脳虚血に対する細胞移植治療におけるペリサイトの果たす役割
| Project/Area Number |
26861169
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
中村 晋之 福岡歯科大学, 歯学部, 助教 (80713742)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Discontinued (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ペリサイト / bFGF / PDGFR-β / 遊走 / 修復 / 線維化 / 脳梗塞 |
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| Outline of Annual Research Achievements |
虚血性傷害時における，脳血管ペリサイトによる幹細胞の制御機構ならびに放出される液性因子について明らかとすることを目的とし，当該年度はペリサイトのkey moleculeであるPDGFRに着目し，細胞遊走に関わる因子の探索を行った．PDGFRの発現は低酸素やアシドーシスといった虚血刺激でなく，虚血によって誘導されるbFGFによって調節される可能性を見いだした．さらに，bFGFはPDGFRを介して増殖および遊走能に関与していた．
ペリサイトにおけるPDGFRシグナルは脳虚血後の修復過程に重要な役割があると考えられ，そのシグナルを調節しうる一つの機序と考えられる．
We found that basic fibroblast growth factor (bFGF), but neither hypoxia nor acidification, significantly upregulated the PDGFRβ expression in human cultured CNS pericytes. The expression of bFGF and FGFR1 was markedly induced in the ischemic hemisphere after ischemic insult in a MCAO stroke model. Immunofluorescent double labeling demonstrated that the expression of bFGF and FGFR1 was co-localized with PDGFRβ-positive cells in peri-infarct areas. Moreover, treatment with bFGF enhanced cell growth and the PDGF-BB-induced migratory activity of cultured pericytes. These data suggest that increased bFGF might upregulate the expression of PDGFRβ, a key molecule of pericytes, and may enhance PDGFRβ-mediated pericyte functions after brain ischemia.
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Report
(1 results)
Research Products
(3 results)
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[Journal Article] Involvement of platelet-derived growth factor receptor β in fibrosis through extracellular matrix protein production after ischemic stroke2015
Author(s)
Makihara N, Arimura K, Ago T, Tachibana M, Nishimura A, Nakamura K, Matsuo R, Wakisaka Y, Kuroda J, Sugimori H, Kamouchi M, Kitazono T
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Journal Title
Experimental Neurology
Volume: 264
Pages: 127-134
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A CADASIL-like case with a novel noncysteine mutation of the NOTCH3 gene and granular deposits in the renal arterioles.2015
Author(s)
Nakamura K, Ago T, Tsuchimoto A, Noda N, Nakamura A, Ninomiya T, Uchiumi T, Tsuruya K, Kamouchi M, Ooboshi H, Kitazono T
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Journal Title
Case Rep Neurol Med
Volume: 2015
Pages: 431461-431461
DOI
Related Report
Peer Reviewed / Open Access
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