| Project/Area Number |
26861170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 悪性脳腫瘍 / グリオーマ / 5-ALA / PGE2 / マクロファージ / 免疫逃避機構 / 脳腫瘍 / プロスタグランジン / 免疫抑制機構 / プロスタグランジンE2 / 放射線治療 / 腫瘍免疫 |
|---|
| Outline of Final Research Achievements |
A subcutaneous tumor model using a glioma cell line (RSV-M) was prepared for C3H / HeN mice and oral administration of 5-aminolevulinic acid (5-ALA) was performed, and tumor growth suppression was confirmed. 5-ALA inhibited the prostaglandin E2 (PGE2) production, which was an immunosuppressive factor in macrophages, and the expression of PGE2 synthetases COX-2 and mPGES-1 was suppressed. When macrophages treated with 5-ALA were co-cultured with RSV-M, the cytotoxic activity was confirmed in a 5-ALA concentration-dependent manner. 5-ALA released the immune escape mechanism of macrophages by PGE2, resulting in anti-glioma effect.
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