a novel therapy targeting miRNA of clear cell sarcoma

• Project/Area Number: 26861182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Gifu University
• Principal Investigator: Nagano Akihito 岐阜大学, 医学部附属病院, 講師 (60422721)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: clear cell sarcoma / miRNA / siRNA / microarray / micro RNA

Outline of Final Research Achievements

Clear cell sarcoma is one of malignant soft tissue sarcoma and EWS/ATF1, chimeric fusion gene, plays important role of tumorigenesis. The objective of this research is to reveal the relationship between EWS/ATF1 and microRNA (miRNA). An siRNA targeting EWS/ATF1 made miR-3127-5p to be upregulated. Transfection of miR-3127-5p to clear cell sarcoma cell line revealed both a suppression of cell proliferation and migratory capacity. These findings suggest miR-3127-5p has a potential to be a novel target of clear cell sarcoma treatment.