The development of innovative therapy for intervertebral disc degeneration targeted to the RANK/RANKL/OPG system

• Project/Area Number: 26861184
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: Takegami Norihiko 三重大学, 医学(系)研究科(研究院), リサーチアソシエイト (20727664)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 椎間板変性 / RANKL / 炎症性サイトカイン

Outline of Final Research Achievements

The present study showed that the expression of RANK/RANKL/OPG system was confirmed both by mRNA and protein levels in the rat and human intervertebral discs.
The expressions of RANK/RANKL/OPG were regulated by stimulation with IL-1β. Those of proinflammatory cytokines and proteolytic enzymes were significantly up-regulated by stimulation with RANKL in the presence of IL-1β stimulation.
The RANK/RANKL/OPG system has the potential to interact with proinflammatory cytokines and be involved in the progression of disc degeneration.

Research Products

• [Presentation] ラット椎間板におけるRANK/RANKL/OPG系の発現と機能解析 (2015)
  • Author(s): 竹上徳彦、明田浩司、村田耕一郎、山田淳一、須藤啓広
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山市
  • Year and Date: 2015-10-22
• [Presentation] ヒト椎間板にRANK/RANKL/OPG系が存在する (2015)
  • Author(s): 竹上徳彦、明田浩司、山田淳一、須藤啓広
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山市
  • Year and Date: 2015-10-22
• [Presentation] Association between RANK/RANKL Signaling and Proinflammatory Cytokines in Rat Intervertebral Disc Cells (2015)
  • Author(s): Norihiko Takegami, Koji Akeda, Koichiro Murata, Junichi Yamada, Akihiro Sudo
  • Organizer: 41th The International Society for the Study of the Lumbar Spine
  • Place of Presentation: SAN FRANCISCO, CA, USA
  • Year and Date: 2015-06-08
  • Int'l Joint Research
• [Presentation] 椎間板組織におけるRANK/RANKL/OPG系の発現とその意義 (2015)
  • Author(s): 竹上徳彦、明田浩司、村田耕一郎、山田淳一、須藤啓広
  • Organizer: 第88回日本整形外科学会学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-05-21
• [Presentation] ラット椎間板におけるRANK/RANKLシグナルと炎症性サイトカインの関連性 (2015)
  • Author(s): 竹上徳彦、明田浩司、村田耕一郎、山田淳一、須藤啓広
  • Organizer: 第44回日本脊椎脊髄病学会学術総会
  • Place of Presentation: 福岡市
  • Year and Date: 2015-04-16
• [Presentation] RANK/RANKL/OPG System in the Rat Intervertebral Disc (2015)
  • Author(s): Norihiko Takegami, Koji Akeda, Koichiro Murata, Junichi Yamada, Akihiro Sudo
  • Organizer: 15th The International Society for Advancement of Spine Surgery
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2015-04-15
  • Int'l Joint Research
• [Presentation] RANK/RANKL/OPG System in the Rat Intervertebral Disc (2015)
  • Author(s): Norihiko Takegami, Koji Akeda, Koichiro Murata, Junichi Yamada, Akihiro Sudo
  • Organizer: Orthopaedic Research Society Annual Meeting 2015
  • Place of Presentation: Las Vegas (U.S.A.)
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] ラット椎間板におけるRANK/RANKL/OPG 系の発現と椎間板変性への関与 (2014)
  • Author(s): 竹上徳彦、明田浩司、村田耕一郎 山田淳一、須藤啓広
  • Organizer: 第22回 日本腰痛学会
  • Place of Presentation: 幕張メッセ (千葉市)
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] ラット椎間板におけるRANK/RANKL/OPG 系の発現とその意義 (2014)
  • Author(s): 竹上徳彦、明田浩司、村田耕一郎、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第29回 日本整形外科学会基礎学術集会
  • Place of Presentation: 城山観光ホテル (鹿児島市)
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] ヒト椎間板組織におけるRANK/RANKL/OPG 系の発現 (2014)
  • Author(s): 竹上徳彦、明田浩司、村田耕一郎、藤原達彦、倉田竜也、近藤哲士、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第29回 日本整形外科学会基礎学術集会
  • Place of Presentation: 城山観光ホテル (鹿児島市)
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] Expression of the RANK/RANKL/OPG system in the rat intervertebral disc (2014)
  • Author(s): Norihiko Takegami, Koji Akeda, Koichiro Murata, Akihiro Sudo
  • Organizer: The International Society for the Study of the Lumbar Spine 2014
  • Place of Presentation: Korea (Seoul)
  • Year and Date: 2014-06-03 – 2014-06-07