| Project/Area Number |
26861185
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Research Collaborator |
IMAI Shinji
MATSUURA Hiroshi
TOYODA Futoshi
MAEDA Tsutomu
URATANI Yoko
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 変形性関節症 / 軟骨細胞 / イオンチャネル / 炎症性サイトカイン / 浸透圧変化 |
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| Outline of Final Research Achievements |
Elucidation of physiological function and construction of detailed cartilage models, including chondrocyte homeostatic maintenance mechanisms may facilitate future OA preventative treatments.
It is clear that mechanical activity of joints couples to chondrocyte metabolic activity, but the role of this coupling in OA is unknown. A simple way to mechanically disturb chondrocytes whilst simultaneously making electrophysiology from chondrocytes is to use osmotic shock. This study aimed to investigate the effects of inflammatory cytokines on functional expression of anion channels in chondrocytes. In this simple model of inflammatory OA we have discovered that an additional phenotype of ion channel becomes evident following cytokine treatment. The identity of these molecular markers is currently unknown and future experiments will use a combination of physiological, pharmacological and molecular biological techniques to identify putative changes in underlying anion channel gene expression.
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