Establishment of treatment strategy against osteoarthritis targeting transcription factor Nkx3.2
| Project/Area Number |
26861189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
Ebina Kosuke 大阪大学, 医学(系)研究科(研究院), 助教 (70612076)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 軟骨再生 / 滑膜細胞 / 変形性関節症 / 関節リウマチ / 同種移植 / 細胞バンク / 同種細胞移植 / 遺伝子導入 |
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| Outline of Final Research Achievements |
We confirmed that gene expression of Nkx3.2 is down-regulated in degenerated cartilage compared to intact cartilage of osteoarthritis patients. We developed scaffold-free tissue engineering construct (TEC) from synovial mesenchymal stem cells (SMSCs) which repairs cartilage defect. We also developed Nkx3.2 gene inserted plasmid, and transfected into synovial MSCs, which showed 90-30000 folds overexpression of Nkx3.2 gene and 2-7 folds of Sox9 gene. Finally, we induced chondrogenic differentiation to this Nkx3.2 gene overexpressed synovial MSCs, while failed to confirm significant increase in size and extra-cellular matrix production. Then, we tried to establish allogenic SMSCs bank to overcome the disadvantage of autogenic transplantation. We investigated that SMSCs from osteoarthritis and rheumatoid arthritis patients exhibit good potential to repair the osteochondral defects of nude rat, indicating the possibility of promising cell sources for cartilage repair.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] The efficacy and safety of additional administration of tacrolimus in patients with rheumatoid arthritis who showed an inadequate response to tocilizumab.2016
Author(s)
Shoichi Kaneshiro, Kosuke Ebina, Makoto Hirao, Hideki Tsuboi, Masataka Nishikawa, Akihide Nampei, Yoshio Nagayama, Koichiro Takahi, Takaaki Noguchi, Hajime Owaki, Jun Hashimoto, Hideki Yoshikawa.
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Journal Title
Mod Rheumatol
Volume: In Press
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-α-induced osteoclastogenesis and promoting osteoblastic differentiation in mice.2015
Author(s)
Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H.
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Journal Title
Biochem Biophys Res Commun
Volume: 465(3)
Issue: 3
Pages: 638-43
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] 1.Semaphorin 4D contributes to rheumatoid arthritis by inducing inflammatory cytokine production: Pathogenic and therapeutic implications2015
Author(s)
Yoshida Y, Ogata A, Kang S, Ebina K, Shi K, Nojima S, Kimura T, Ito D, Morimoto K, Nishide M, Hosokawa T, Hirano T, Shima Y, Narazaki M, Tsuboi H, Saeki Y, Tomita T, Tanaka T, Kumanogoh A.
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Journal Title
Arthritis Rheumatol
Volume: none
Issue: 6
Pages: 1481-1490
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility2014
Author(s)
Jin H, Arase N, Hirayasu K, Kohyama M, Suenaga T, Saito F, Tanimura K, Matsuoka S, Ebina K, Shi K, Toyama-Sorimachi N, Yasuda S, Horita T, Hiwa R, Takasugi K, Ohmura K, Yoshikawa H, Saito T, Atsumi T, Sasazuki T, Katayama I, Lanier LL, Arase H
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Journal Title
Proceedings of National Academy of Science USA
Volume: 111
Issue: 10
Pages: 3787-3792
DOI
Related Report
Peer Reviewed / Open Access
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