| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
We previously constructed a fusion protein consisting (CB-bFGF) of bFGF and the collagen binding domain (CBD) of Clostridium histolyticum collagenase and revealed that CB-bFGF markedly enhances bone formation in mouse femoral fracture model, suggesting that the combination of CB-bFGF with an collagen materials would promote vertebral fracture. Here, to attempt to apply the bone formation seeds to the vertebral fracute repair, we developed an injectable collagen powder (CP)/ CB-bFGF and demineralized bone powder (DBP)/CB-bFGF composite. CP/ CB-bFGF and BP/CB-bFGF composite accelerates bone formation in a vertebral bone defect model in rat and clearly showed that the composite also increases bone mineral density at fracture sites compared to CP and DBP alone. Our results suggest that CP/ CB-bFGF and DBP/CB-bFGF may be useful for promoting vertebral fracture healing in the clinical setting.
|
