Investigation of vertebral compression fracture healing methods

• Project/Area Number: 26861209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: Saito Wataru 北里大学, 医学部, 助教 (60439099)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 椎体骨折 / 塩基性線維芽細胞増殖因子 / コラーゲン結合ドメイン / コラーゲン結合型成長因子 / 骨折治療

Outline of Final Research Achievements

We previously constructed a fusion protein consisting (CB-bFGF) of bFGF and the collagen binding domain (CBD) of Clostridium histolyticum collagenase and revealed that CB-bFGF markedly enhances bone formation in mouse femoral fracture model, suggesting that the combination of CB-bFGF with an collagen materials would promote vertebral fracture. Here, to attempt to apply the bone formation seeds to the vertebral fracute repair, we developed an injectable collagen powder (CP)/ CB-bFGF and demineralized bone powder (DBP)/CB-bFGF composite. CP/ CB-bFGF and BP/CB-bFGF composite accelerates bone formation in a vertebral bone defect model in rat and clearly showed that the composite also increases bone mineral density at fracture sites compared to CP and DBP alone. Our results suggest that CP/ CB-bFGF and DBP/CB-bFGF may be useful for promoting vertebral fracture healing in the clinical setting.

Research Products

• [Journal Article] Effect of Freeze-Dried Allograft Bone with Human Basic Fibroblast Growth Factor Containing a Collagen-Binding Domain From Clostridium Histolyticum Collagenase on Bone Formation After Lumbar Posterolateral Fusion Surgery in Rats (2017)
  • Author(s): Inoue G, Uchida K, Matsushita O, Fujimaki H, Saito W, Miyagi M, Sekiguchi H, Nishi N, Ohtori S, Yogoro M, Takaso M.
  • Journal Title: Spine Volume: 印刷中 Issue: 17 Pages: 995-1001
  • DOI: 10.1097/brs.0000000000002074
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spinal Epidural Hematoma after Thoracolumbar Posterior Fusion Surgery without Decompression for Thoracic Vertebral Fracture. (2016)
  • Author(s): Minato T, Miyagi M, Saito W, Shoji S, Nakazawa T, Inoue G, Imura T, Minehara H, Matsuura T, Kawamura T, Namba T, Takahira N, Takaso M.
  • Journal Title: Case Rep Orthop Volume: - Pages: 6295817-6295817
  • DOI: 10.1155/2016/6295817
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Common iliac vein injury during L5-S1 posterior lumbar interbody fusion in a patient with systemic lupus erythematosus receiving steroid treatment: A case report. (2016)
  • Author(s): Saito W, Inoue G, Nakazawa T, Imura T, Miyagi M, Uchida K, Namba T, Shirasawa E, Takahira N, Takaso M.
  • Journal Title: J Orthop Sci. Volume: - Issue: 5 Pages: 962-966
  • DOI: 10.1016/j.jos.2015.12.011
  • Peer Reviewed
• [Journal Article] Acceleration of bone formation during fracture healing by poly(pro-hyp-gly)10 and basic fibroblast growth factor containing polycystic kidney disease and collagen-binding domains from Clostridium histolyticum collagenase (2016)
  • Author(s): Sekiguchi H, Uchida K, Inoue G, Matsushita O, Saito W, Aikawa J, Tanaka K, Fujimaki H, Miyagi M, Takaso M.
  • Journal Title: J Biomed Mater Res A Volume: 104 Issue: 6 Pages: 1372-1378
  • DOI: 10.1002/jbm.a.35670
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Acceleration of callus formation during fracture healing using basic fibroblast growth factor-kidney disease domain-collagen binding domain fusion protein combined with allogenic demineralized bone powder. (2015)
  • Author(s): Saito W, Uchida K, Matsushita O, Inoue G, Sekiguchi H, Aikawa J, Fujimaki H, Takaso M.
  • Journal Title: J Orthop Surg Res Volume: -
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Acceleration Of Bone Repair During Fracture Healing By Poly(pro-hyp-gly)10 And Basic Fibroblast Growth Factor Containing Collagen-binding Domains From Clostridium Histolyticum Collagenase (2017)
  • Author(s): Sekiguchi H, Uchida K, Inoue G, Saito W, Aikawa J, Fujimaki H, Nagura N, Takano S, Miyagi M Takaso M
  • Organizer: Orthopaedic Research Society 2017 Annual Meeting
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] Bone Powder とコラーゲン結合型線維芽細胞増殖因子による骨折後の骨形成促進効果の検討 (2014)
  • Author(s): 斎藤 亘、内田健太郎、井上玄、松下治、大貫裕子、藤巻寿子、関口裕之、相川 淳、高平尚伸、高相晶士.
  • Organizer: 第29回日本整形外科学会基礎学術集会
  • Place of Presentation: 城山観光ホテル（鹿児島県鹿児島市）
  • Year and Date: 2014-10-09 – 2014-10-10