| Project/Area Number |
26861217
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kindai University |
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Principal Investigator |
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| Research Collaborator |
ASAHARA Hiroshi 東京医科歯科大学, 医学部, 教授
Lotz Martin スクリプス研究所, 分子実験医学部門, 教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 再生医療 / 間葉系幹細胞 / リプログラミング / ダイレクトリプログラミング / 上皮間葉転換 / 転写因子 / Twist1 / TGFβシグナル / iPS細胞 |
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| Outline of Final Research Achievements |
Mesenchymal stem cells (MSCs) are multipotent stem cells that are present in multiple tissues. Recent studies demonstrated that MSCs can support wound healing and tissue regeneration. On the other hand, adult MSCs exhibit cellular senescence during ex vivo expansion, which is accompanied by a reduction in self-renewal and multidifferentiation potential. To resolve this, identification of master molecules and central cascades involving MSC self-renewal is essential. Here, we focused on Twist1, which is a master regulator of epithelial-mesenchymal transition (EMT), and examined it’s roles in MSCs. Suppression of Twist1 leaded to reduction of Sox2, Bmi1, and Gata6 expressions. Contrary, over-expression of Twist1 induced expression of these genes. Mass spectrometric analysis showed that Tiwist1 involves epigenetic modification. Finally, we demonstrated that direct induction of MSCs from human monocytes was possible by introduce Twist1 with reprogramming factors.
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