| Project/Area Number |
26861235
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Research Collaborator |
KAKIHANA Yasuyuki 鹿児島大学, 医歯学域医学系, 教授 (20264426)
YASUDA Tomotsugu 鹿児島大学, 医歯学域附属病院, 講師 (80437954)
ITOU Takashi 鹿児島大学, 医歯学総合研究科, 講師 (20381171)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 虚血再灌流障害 / ヒストン / 細胞外ヒストン / 麻酔 / 虚血再灌流傷害 / 虚血再灌流 |
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| Outline of Final Research Achievements |
Ischemia-reperfusion injury occurs during a surgical procedure, such as liver resection, organ transplantation, and cardiovascular surgery. Moreover, it leads to severe tissue dysfunction and organ failure. Recent studies have shown that histones are released into the extracellular space during sepsis, trauma, and ischemia-reperfusion injury, and act as major mediators of the death of an organism. We investigated the correlation between extracellular histones and ischemia-reperfusion injury during liver resection and lower limb ischemia mouse model. This study showed the trends of the circulating histone H3 levels increase after ischemia-reperfusion and were related to ischemia time. We examined whether circulating histones are released from necrotic cells or neutrophils with cecal ligation and puncture(CLP)-induced sepsis. We also found that circulating histone H3 levels in septic conditions are mainly derived from neutrophils rather than damaged cells.
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