Mechanism of metastasis of bladder cancer cells via decreased expression of delta N p63 caused by adhesion to extracellular matrix

• Project/Area Number: 26861268
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: Yoshida Takahiro 大阪大学, 医学(系)研究科(研究院), 寄附講座助教 (80583624)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 膀胱癌 / 転移 / p63 / 細胞外基質 / delta N p63 alpha / cell cluster / cell adhesion / eextracellular matrix / urothelial cancer

Outline of Final Research Achievements

The dissemination of bladder cancer cells is supposed to be a major cause of multicentricity of bladder cancer. Clarifying mechanism of the dissemination can lead to development of new therapy to suppress intravesical relapse. We discovered that ΔNp63α was rapidly degraded when clusters of bladder cancer cells attached to extracellular matrix. We originally developed experimental model which mimic the dissemination of cancer cells. Using this experimental model, degradation of ΔNp63α was shown to be involved in the dissemination.

Research Products

• [Journal Article] Dynamic Change in p63 Protein Expression during Implantation of Urothelial Cancer Clusters. (2015)
  • Author(s): Yoshida T, Okuyama H, Nakayama M, Endo H, Tomita Y, Nonomura N, Nishimura K, Inoue M.
  • Journal Title: Neoplasia Volume: 17 Issue: 7 Pages: 574-585
  • DOI: 10.1016/j.neo.2015.07.004
  • Peer Reviewed / Open Access
• [Presentation] 尿路上皮癌の尿路腔内播種におけるΔNp63αの関与 (2015)
  • Author(s): 吉田栄宏、奥山裕照、井上正宏
  • Organizer: 第24回がん転移学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-07-23
• [Presentation] 膀胱癌腔内播種in vitro実験モデルを用いた腔内播種メカニズムの解明： ΔNp63αの分解は膀胱癌の腔内播種に関与する (2015)
  • Author(s): 吉田栄宏、中山雅志、奥山裕照、西村和郎、井上正宏、野々村祝夫
  • Organizer: 第103回日本泌尿器科学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-04-18
• [Presentation] 膀胱癌腔内播種におけるΔNp63αの関与 (2015)
  • Author(s): 吉田栄宏、奥山裕照、中山雅志、西村和郎、井上正宏、野々村祝夫
  • Organizer: 第24回泌尿器分子・細胞研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-18
• [Presentation] 尿路上皮癌細胞塊の細胞外基質への接着はdelta N p63の速やかな分解を誘発する (2014)
  • Author(s): 吉田栄宏、奥山裕照、中山雅志、野々村祝夫、西村和郎、井上正宏
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-27