| Project/Area Number |
26861269
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腎移植 / 急性細胞性拒絶反応 / バイオマーカー / ケモカイン / 移植・再生医療 / 拒絶反応 |
|---|
| Outline of Final Research Achievements |
In the organ transplantation, it is important that I make an early diagnosis of T-cell mediated rejection (TCMR) non-invasively. We paid attention to chemokines. We made rat allograft model of renal transplantation and quantitative real-time PCR revealed that the expression of CCL2, CCL3, CCL5, CXCL9 and CXCL10 significantly was increased in the rat allograft from an early stage. In the human TCMR cases diagnosed by the graft biopsy, quantitative flow cytometry results showed that serum CXCL9, CXCL10 and CCL5 significantly elevated in comparison with the non-TCMR, and serum CXCL10 was suitable for a diagnosis prediction of TCMR by ROC curve. These results demonstrate that serum CXCL10 concentration may be a highly accurate biomarker for predicting TCMR.
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