| Project/Area Number |
26861278
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 尿路感染症 / 尿路病原性大腸菌 / サーファクタントタンパク質A / ウロプラキン |
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| Outline of Final Research Achievements |
We demonstrated that surfactant protein A (SP-A)-deficient mice were more susceptible to uropathogenic Escherichia coli (UPEC) infection than WT mice. SP-A directly bound to UPEC in a Ca2+-dependent manner. SP-A inhibited growth of UPEC in human urine. The binding of SP-A to UPEC decreased the adherence of bacteria to urothelial cells. These results indicate that direct action of SP-A on UPEC is important in host defense against UPEC. In addition, adhesion of UPEC to urothelial cells was decreased when urothelial cells were preincubated with SP-A. Adhesion of UPEC to urothelial cells is achieved via interaction between FimH, an adhesin located at bacterial pili, and uroplakin Ia, a glycoprotein expressed on the urothelium. SP-A directly bound to uroplakin Ia and competed with FimH for uroplakin Ia binding. Our data clearly indicate that SP-A plays indispensable roles in host defense against UPEC.
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