Elucidation of novel molecular mechanisms in androgen independent growth of prostate cancer
Project/Area Number |
26861280
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Urology
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Research Institution | Yokohama City University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 前立腺癌 / miRNA / CRPC / 再燃癌 |
Outline of Final Research Achievements |
We are interested in molecular mechanisms involved in the progression of castration-resistant prostate cancer (CRPC). We found significantly higher expression of miR-30d in the androgen-independent LNCaP-AI cell line compared with the androgen-dependent LNCaP cell line using miRNA microarrays and qPCR. Clinicopathological study revealed that miR-30d expression levels were significantly higher in bone metastatic CRPC tissue than in untreated primary prostate cancer tissue. To evaluate the biological functions of miR-30d during the progression of CRPC, we determined whether miR-30d overexpressing cell lines grew well without androgen, and thus manifest growth characteristics of CRPC. As shown by the cell proliferation assay, LNCaP-30d cell lines were able to grow in an androgen-indepleted medium, while the cotrol cell (LNCaP-C) grew poorly in the absence of androgen. Taken together, we found that miR-30d promotes prostate cancer cell proliferation in an androgen-indepleted.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Neutrophil-to-lymphocyte ratio predicts prostatic carcinoma in men undergoing needle biopsy.2015
Author(s)
Kawahara T, Fukui S, Sakamaki K, Ito Y, Ito H, Kobayashi N, Izumi K, Yokomizo Y, Miyoshi Y, Makiyama K, Nakaigawa N, Yamanaka T, Yao M, Miyamoto H, Uemura H
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Journal Title
Oncotarget
Volume: 6(31)
Issue: 31
Pages: 32169-32176
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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