| Project/Area Number |
26861281
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Yokohama City University |
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Principal Investigator |
KOMEYA Mitsuru 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (60721082)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 男性不妊 / 精子形成 / マイクロ流体 / 組織培養 |
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| Outline of Final Research Achievements |
The causes of male infertility are mostly attributed to the dysfunction of spermatogenesis. However, the mechanism and treatment of spermatogenetic failure are not well revealed. The achievement of human in vitro spermatogenesis will be a powerful tool to reveal the mechanism of male infertility. In 2011, we improved the air-liquid interphase method and achieved in vitro spermatogenesis in mice. The durability and efficiency of spermatogenesis were low in the culture system. Therefore, we imitated the in vivo micro-environment using the microfluidic technology which was derived from the technique manufacturing the semiconductor. The microfluidic device maintained the two major functions of the testis, spermatogenesis and hormone production, for more than 6 months. It will enable us to disclose the mechanism of spermatogenesis precisely mimicking in vivo condition.
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