| Project/Area Number |
26861297
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Keio University |
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Principal Investigator |
OBATA JUN 慶應義塾大学, 医学部, 助教 (20570865)
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| Research Collaborator |
MIYAJIMA AKIRA 慶應義塾大学, 医学部, 准教授 (90245572)
OYA MOTOTSUGU 慶應義塾大学, 医学部, 教授 (00213885)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 脂肪酸受容体 / 肥満 / 腎細胞癌 / 抗腫瘍効果 |
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| Outline of Final Research Achievements |
In renal cell carcinoma, we evaluated the impact of GPR120, a fatty acid receptor which has been identified as the important substance associated with metabolic syndrome. For examining short-term antitumor effect by stimulating GPR120, cell viability was measured subsequently after the exposure of agonists using WST-assay in a variety of renal cell carcinoma cell lines. We observed no obvious inhibition of proliferation and no change of GPR120 activity over time by western blot method. Although we also evaluated the long-term cytotoxicity by regular GPR120 stimulation, we found no remarkable change concerning the cellular proliferative potential and expression of GPR120 by western blot analysis regardless of conditions. Because it is expected that GPR120 is significantly associated with cancer for its anti-inflammatory effects, processes and results of our study under various conditions may be helpful for future study.
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