| Project/Area Number |
26861320
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Research Collaborator |
ORISAKA MAKOTO 福井大学, 学術研究院医学系部門(附属病院部), 講師 (80324143)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 卵胞 / 莢膜細胞 / 顆粒膜細胞 / 人口卵胞壁モデル / LH / アポトーシス / IGF / E2 / LH受容体 / 卵巣 / 卵胞の選択と閉鎖 / ＩＧＦ / アンドロゲン / パラクライン調節 / エストロゲン / FSHレセプター / LHレセプター |
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| Outline of Final Research Achievements |
Only a single dominant follicle can ovulate in the human menstrual cycle, whereas most follicles undergo atresia by granulosa cell apoptosis, a selection process that ensures the release of only the healthiest and most viable oocyte. The mechanism during selection of a dominant follicle is still unknown. In this study, we isolated granulosa cells and theca cells from bovine small follicles, and cultured them in an in vitro “two-cell two-gonadotropin” system. Luteinizing hormone (LH) stimulation to theca cells induced the expressions of LH receptor and aromatase in granulosa cells. LH stimulation to theca cells also increased estrogen production and suppressed apoptosis in granulosa cells. These results suggest that LH stimulation to theca cell plays a critical role during the follicle selection from the follicle stimulating hormone (FSH)-dependent growth of early follicles to the LH-dependent maturation of a dominant follicle.
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