| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Immunohistochemistry revealed that C2GnT was highly expressed in trophoblasts of choriocarcinoma but not in hydatidiform mole. Expression level of C2GnT protein in siRNA transfectants were significantly reduced compared to WT and controls at 24 hours after transfection. MTS assay showed no effect on cell proliferation in Jar for 72 hours after C2GnT siRNA transfection. Suppression of C2GnT expression reduced migration and invasive abilities significantly. The rate of cell attachment to fibronectin, collagen type I, and collagen type IV were decreased by C2GnT knockdown.
These findings suggest that C2GnT may be involved in regulating malignant potential of trophoblasts by intracellular expression.
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