Identification of fetal-specific microRNAs as potential marker for amniotic fluid embolism

• Project/Area Number: 26861333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: HIGASHIJIMA Ai 長崎大学, 医歯薬学総合研究科(医学系), 助教 (00549595)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 産婦人科 / 羊水塞栓症 / microRNA / 母体血漿 / 胎児 / 胎盤 / C14MC / 胎児得的 / cell-free / exosome / 羊水穿刺 / 母児間輸血 / 次世代高速シークエンス / 胎児特異的

Outline of Final Research Achievements

Fifteen fetal blood cell-specific miRNAs were identified. Fourteen out of them located on chromosome 14 microRNA cluster region (C14MC; e.g. miR-370, miR-485, miR-432 etc), and remaining is miR-452 located on Xq-28. Micro RNAs on C14MC were highly expressed in cord blood cell, newborn blood cell and placental tissue, though microRNAs on C19MC were expressed only in placental tissue. By comprehensive analysis, microRNAs expressed highly in fetal blood cells than maternal blood cells were identified. Most of them located on C14MC region, and may be candidate molecular marker for amniotic fluid embolism. In addition, plasma concentrations of cell-free miR-323-3p could distinguish EP from SA yielding an area under the curve of 0.7454 (95% confidence interval: 0.5558-0.9349).

Research Products

• [Journal Article] Increased levels of cell-free miR-517a and decreased levels of cell-free miR-518b in maternal plasma samples from placenta previa pregnancies at 32 weeks gestation (2015)
  • Author(s): Hasegawa Y, Miura K, Higashijima A, Abe S, Miura S, Yoshiura KI, Masuzaki H
  • Journal Title: Reproductive Sciences Volume: 22 Pages: 1569-1576
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Circulating levels of C19MC-cluster microRNAs in pregnant women with severe pre-eclampsia (2015)
  • Author(s): Miura K, Higashijima A, Murakami Y, Tsukamoto O, Hasegawa Y, Abe S, Naoki Fuchi N, Miura S, Kaneuchi M, Masuzaki H
  • Journal Title: The Journal of Obstetrics and Gynaecology Research Volume: 41 Pages: 1526-1532
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy (2015)
  • Author(s): Miura K, Higashijima A, Mishima H, Miura S, Kitajima M, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Fertility and Sterility Volume: 103 Pages: 1202-1208
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Circulating levels of maternal plasma cell-free miR-21 are associated with maternal body mass index and neonatal birth weight (2015)
  • Author(s): Miura K, Higashijima A, Hasegawa Y, Abe S, Miura S, Fuchi N, Murakami Y, Kinoshita A, Yoshida A, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Prenatal Diagnosis Volume: 35 Pages: 509-511
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of labor on plasma concentrations and postpartum clearance of cell-free, pregnancy-associated, placenta-specific microRNAs (2015)
  • Author(s): Morisaki S, Miura K, Higashijima A, Abe S, Miura S, Hasegawa Y, Yoshida A, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Prenatal Diagnosis Volume: 35 Pages: 44-50
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 胎盤機能の評価 (2015)
  • Author(s): 東島 愛、増崎英明
  • Journal Title: 臨床婦人科産科 Volume: 69 Pages: 246-254
• [Journal Article] TTTSレーザー治療の問題：その1「羊膜穿破」 (2015)
  • Author(s): 東島愛、増崎英明
  • Journal Title: ペリネイタルケア Volume: 34 Pages: 647-651
  • Peer Reviewed
• [Journal Article] Effect of labor on plasma concentrations and postpartum clearance of cell-free, pregnancy-associated, placenta-specific microRNAs. (2015)
  • Author(s): Morisaki S, Miura K, Higashijima A, Abe S, Miura S, Hasegawa Y, Yoshida A, Kaneuchi M, Yoshiura KI, Masuzaki H.
  • Journal Title: Prenatal Diagnosis. Volume: 35 Pages: 44-50
  • Peer Reviewed
• [Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome. (2014)
  • Author(s): Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H.
  • Journal Title: Prenatal Diagnosis. Volume: 34 Pages: 345-349
  • Peer Reviewed
• [Journal Article] Identification of Endometrioid Endometrial Carcinoma-associated microRNAs in Tissue and Plasma. (2014)
  • Author(s): Tsukamoto O, Miura K, Mishima H, Abe S, Kaneuchi M, Higashijima A, Miura S, Kinoshita A, Yoshiura KI, Masuzaki H.
  • Journal Title: Gynecologic Oncology Volume: 132 Pages: 715-721
  • Peer Reviewed
• [Journal Article] Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US. (2014)
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Yoshida A, Kaneuchi M, Yoshiura KI and Masuzaki H.
  • Journal Title: Journal of Human Genetics. Volume: 59 Pages: 251-255
  • Peer Reviewed
• [Journal Article] Clinical applications of analysis of plasma circulating complete hydatidiform mole pregnancy-associated miRNAs in gestational trophoblastic neoplasia: A preliminary investigation. (2014)
  • Author(s): Miura K, Hasegawa Y, Abe S, Higashijima A, Miura S, Mishima H, Kinoshita A, Kaneuchi M, Yoshiura KI, Masuzaki H.
  • Journal Title: Placenta. Volume: 35 Pages: 787-789
  • Peer Reviewed
• [Journal Article] Circulating levels of maternal plasma cell-free pregnancy-associated placenta-specific microRNAs are associated with placental weight. (2014)
  • Author(s): Miura K, Morisaki S, Abe S, Higashijima A, Hasegawa Y, Miura S, Tateishi S, Mishima H, Yoshiura K, Masuzaki H.
  • Journal Title: Placenta. Volume: 35 Pages: 848-851
  • Peer Reviewed
• [Journal Article] 常位胎盤早期剥離-発症予知と対策-（超音波診断） (2014)
  • Author(s): 東島 愛、増崎 英明
  • Journal Title: 産婦人科の実際 Volume: 63 Pages: 1951-1957
• [Presentation] 妊婦健診未受診妊婦に発症した周産期心筋症の一例 (2015)
  • Author(s): 東島愛、高原靖、梶村慈、銕尾聡子、野々下晃子、江崎裕幸、神戸太郎、角至一郎、槙田徹次、布施正樹、村上誠、中山大介、増崎英明
  • Organizer: 第38回日本母体胎児医学会学術集会
  • Place of Presentation: ビーコンプラザ、別府市
  • Year and Date: 2015-10-29
• [Presentation] Identification of Endometrioid Endometrial Carcinoma-associated microRNAs in Tissue and Plasma (2015)
  • Author(s): Higashijima A, Miura K, Tsukamoto O, Mishima H, Abe S, Miura S, Kaneuchi M, Kinoshita A, Yoshiura K, Masuzaki H
  • Organizer: 19th International meeting of the European Society of Gynaecological Oncology
  • Place of Presentation: Nice, France
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] 小脳低形成を伴う滑脳症の一例 (2015)
  • Author(s): 東島愛、角至一郎、銕尾聡子、大園恵梨子、大塚純一、神戸太郎、村上誠、増崎英明
  • Organizer: 第51回日本周産期・新生児医学会学術集会
  • Place of Presentation: ヒルトン福岡シーホーク、福岡市
  • Year and Date: 2015-07-10
• [Presentation] 異所性妊娠における母体血漿中胎盤特異的microRNA流入量の臨床的意義に関する検討 (2015)
  • Author(s): 東島愛、三浦清徳、長谷川ゆり、原田亜由美、北島道夫、増崎英明
  • Organizer: 第67回日本産婦人科学会学術講演会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2015-04-09
• [Presentation] 母体血と比較して胎児血で高発現するmicroRNAの同定 (2014)
  • Author(s): 東島愛、三浦清徳、三島博之、木下晃、塚本大空、阿部修平、長谷川ゆり、吉田敦、吉浦孝一郎、増崎英明
  • Organizer: 日本人類遺伝学会第59回大会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2014-11-19 – 2014-11-22
• [Presentation] 母体血と比較して胎児血で高発現するmicroRNAの同定 (2014)
  • Author(s): 東島愛、三浦清徳、塚本大空、阿部修平、長谷川ゆり、吉田敦、増崎英明
  • Organizer: 第37回日本母体胎児学会学術集会
  • Place of Presentation: ハウステンボス（長崎県佐世保市）
  • Year and Date: 2014-11-07 – 2014-11-08
• [Presentation] 母体血と比較して胎児血で高発現するmicroRNAの同定 (2014)
  • Author(s): 東島愛、三浦清徳、塚本大空、阿部修平、長谷川ゆり、吉田敦、増崎英明
  • Organizer: 第66回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2014-04-18 – 2014-04-20
• [Remarks] 長崎大学病院産婦人科
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/
• [Remarks] 長崎大学医学部産婦人科ホームページ
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/