Reconstruction of the uterus through differentiation of induced pluripotent stem cells within decellularized uterine matrix
| Project/Area Number |
26861340
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生殖医学 / 組織再構築 / 脱細胞化 / 再細胞化 / iPS細胞 / ミュラー管 |
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| Outline of Final Research Achievements |
Rat iPS cells (riPSCs) were purchased and propagated. GFP gene and the luciferase gene were introduced in riPSCs with the lentiviral vector. GFP-positive riPSCs were cloned by single cell sorting of GFP-positive cells using FACS. GFP-positive riPSCs were transplanted into immunodeficient mice subcutaneously, and the cellular luminescence, which was generated by degradation of luciferin catalyzed by luciferase, and reflects the cell growth, was non-invasively tracked using in vivo imaging system. Teratoma formation was confirmed 6weeks after the transplantation.
RiPSCs were cultured in a medium supplemented with several growth factors for differentiation. Quantitative real-time PCR was performed to confirm the expression of the gene markers, and the results suggested that riPSCs underwent differentiation into Mullerian lineage via intermediate mesoderm.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] CD34 and CD49f double-positive and lineage marker-negative cells isolated from human myometrium exhibit stem cell-like properties involved in pregnancy-induced uterine remodeling.2015
Author(s)
Ono M, Kajitani T, Uchida H, Arase T, Oda H, Uchida S, Ota K, Nagashima T, Masuda H, Miyazaki K, Asada H, Hida N, Mabuchi Y, Morikawa S, Ito M, Bulun SE, Okano H, Matsuzaki Y, Yoshimura Y, Maruyama T
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Journal Title
Biol Reprod
Volume: 93巻
Issue: 2
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Non-invasive and real-time assessment of peritoneal lesions generated from human endometrial cell transplants locally accumulated by magnetic force in immunodeficient mice.2015
Author(s)
Tetsuo Maruyama, Hirotaka Masuda, Naoko Hida, Hideyuki Oda, Kaoru Miyazaki, Miki Fumie, Hanako Hihara, Kuniaki Ota, Hiroshi Uchida, Mamoru Tanaka
Organizer
31th Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE).
Place of Presentation
Lisbon, Portugal
Year and Date
2015-06-12
Related Report
Int'l Joint Research
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[Presentation] 頸管拡張を必要としない子宮鏡下ポリープ切除術．2014
Author(s)
升田博隆, 佐藤健二, 佐藤 卓, 太田邦明, 三木史恵, 宮崎 薫, 内田明花, 各務真紀, 内田 浩, 丸山哲夫, 吉村泰典, 田中 守
Organizer
第54回日本産科婦人科内視鏡学会
Place of Presentation
城山観光ホテル（鹿児島県鹿児島市）
Year and Date
2014-09-11 – 2014-09-13
Related Report
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[Presentation] 子宮内膜症から発生したと推察された癌肉腫の一例．2014
Author(s)
吉丸真澄, 仲村 勝, 栗原朋子, 布施由紀子, 宮田あかね, 宮崎 薫, 小川真里子, 吉田丈児, 高松 潔
Organizer
第56回日本婦人科腫瘍学会学術講演会
Place of Presentation
栃木県総合文化センター（栃木県宇都宮市）
Year and Date
2014-07-17 – 2014-07-19
Related Report
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