| Project/Area Number |
26861340
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生殖医学 / 組織再構築 / 脱細胞化 / 再細胞化 / iPS細胞 / ミュラー管 |
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| Outline of Final Research Achievements |
Rat iPS cells (riPSCs) were purchased and propagated. GFP gene and the luciferase gene were introduced in riPSCs with the lentiviral vector. GFP-positive riPSCs were cloned by single cell sorting of GFP-positive cells using FACS. GFP-positive riPSCs were transplanted into immunodeficient mice subcutaneously, and the cellular luminescence, which was generated by degradation of luciferin catalyzed by luciferase, and reflects the cell growth, was non-invasively tracked using in vivo imaging system. Teratoma formation was confirmed 6weeks after the transplantation.
RiPSCs were cultured in a medium supplemented with several growth factors for differentiation. Quantitative real-time PCR was performed to confirm the expression of the gene markers, and the results suggested that riPSCs underwent differentiation into Mullerian lineage via intermediate mesoderm.
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