Role of microglia in functional hypothalamic hypogonadism
| Project/Area Number |
26861345
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
Fujioka Hitomi 聖マリアンナ医科大学, 医学部, 講師 (50410064)
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| Research Collaborator |
AKEMA Tatsuo 聖マリアンナ医科大学, 医学部, 教授 (60128585)
FUNABASHI Toshiya 聖マリアンナ医科大学, 医学部, 教授 (70229102)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | LH / ミクログリア / マクロファージ / ストレス / IL-1β / 終板脈絡器官 / 中枢性性腺機能低下 / GnRH |
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| Outline of Final Research Achievements |
Inflammatory/immune challenge is known to suppress luteinizing hormone (LH) secretion. We previously observed that pretreatment with minocycline, a potent inhibitor of microglial activation, significantly alleviate the suppression by lipopolysaccharide (LPS) of ovarian steroid-induced LH surge in ovariectomized (OVX) rats. In this study, we observed that IL-1β and IL-6, but not IL-2, gene expressions were increased by LPS treatment in the preoptic area of ovarian steroid-primed OVX rats. Minocycline pretreatment tended to attenuate the induction of cytokines by LPS. IL-1β immunoreactivity was found only in Iba1 (a marker of microglia/macrophage) immunoreactive cell in the organum vasculosum of the lamina terminalis (OVLT) in LPS treated rats. These results suggest that IL-1β produced by microglia/macrophage in the OVLT is involved in the inflammatory/immune challenge induced suppression of LH secretion.
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Report
(3 results)
Research Products
(6 results)
