The gene tartgeting therapy with the use of sgRNA targeting cyclin D1 for head and neck cancer

• Project/Area Number: 26861352
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Hokkaido University
• Principal Investigator: Sakashita Tomohiro 北海道大学, 医学研究科, 助教 (30455654)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 頭頸部癌 / cyclin D1 / 遺伝子標的治療 / cyclin D1標的RNA / cyclin D1標的sgRNA / 頭頸部外科学 / サイクリンD1

Outline of Final Research Achievements

I proved that the efficacy of the gene targeting therapy with the use of the sg RNA targeting cyclin D1 mRNA. It was confirmed that the depression of cyclin D1 expression reduced the proliferating potential of tumor cells. The clinical use of the gene targeting therapy with the use of the sg RNA targeting cyclin D1 mRNA may improve the prognosis of patients with cyclin D1 highly expressive head and neck cancer. It may be possible that this treatment be one of the novel therapies against head and neck cancer.

Research Products

• [Journal Article] Comparison of hearing outcomes after treatment for early-stage external auditory canal cancer. (2016)
  • Author(s): Morita S, Nakamaru Y, Homma A, Sakashita T, Hatakeyama H, Masuya M, Fukuda S.
  • Journal Title: Head Neck. Volume: 38 Suppl 1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effectiveness of superselective intra-arterial chemoradiotherapy targeting retropharyngeal lymph node metastasis. (2016)
  • Author(s): Suzuki T, Sakashita T, Homma A, Hatakeyama H, Kano S, Mizumachi T, Yoshida D, Fujima N, Onimaru R, Tsuchiya K, Yasuda K, Shirato H, Suzuki F, Fukuda S.
  • Journal Title: Eur Arch Otorhinolaryngol. Volume: 273 Pages: 3331-3336
  • NAID: 120006347189
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The role of prophylactic neck dissection and tumor thickness evaluation for patients with cN0 tongue squamous cell carcinoma. (2016)
  • Author(s): Tsushima N, Sakashita T, Homma A, Hatakeyama H, Kano S, Mizumachi T, Kakizaki T, Suzuki T, Fukuda S.
  • Journal Title: Eur Arch Otorhinolaryngol. Volume: 273 Pages: 3987-3992
  • NAID: 120006360053
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Outcomes of Surgery and Chemoradiotherapy for Temporal Bone Cancer. (2016)
  • Author(s): Morita S, Homma A, Nakamaru Y, Sakashita T, Hatakeyama H, Kano S, Fukuda A, Fukuda S.
  • Journal Title: Otol Neurotol. Volume: 37 Pages: 1174-1182
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A retrospective study of G-tube use in Japanese patients treated with concurrent chemoradiotherapy for hypopharyngeal cancer. (2016)
  • Author(s): Homma A, Hatakeyama H, Mizumachi T, Kano S, Sakashita T, Kuramoto R, Nakamaru Y, Onimaru R, Tsuchiya K, Yoshida D, Yasuda K, Shirato H, Fukuda S.
  • Journal Title: PLoS One Volume: 11
  • DOI: 10.1371/journal.pone.0161734
  • NAID: 120005866840
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comparison of acute toxicities associated with cetuximab-based bioradiotherapy and platinum-based chemoradiotherapy for head and neck squamous cell carcinomas: A single-institution retrospective study in Japan. (2015)
  • Author(s): Sakashita T, Homma A, Hatakeyama H, Furusawa J, Kano S, Mizumachi T, Iizuka S, Onimaru R, Tsuchiya K, Yasuda K, Shirato H, Fukuda S.
  • Journal Title: Acta Otolaryngol. Volume: 135 Pages: 853-858
  • DOI: 10.3109/00016489.2015.1030772
  • NAID: 120005820705
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clinical outcomes of weekly cisplatin chemoradiotherapy for patients with pyriform sinus cancer. (2015)
  • Author(s): Sakashita T, Homma A, Hatakeyama H, Furusawa J, Kano S, Mizumachi T, Iizuka S, Onimaru R, Tsuchiya K, Yasuda K, Shirato H, Fukuda S.
  • Journal Title: Int J Clin Oncol. Volume: 20 Pages: 1081-1085
  • DOI: 10.1007/s10147-015-0821-8
  • NAID: 120005890011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of initial neck dissection for patients with node-positive oropharyngeal squamous cell carcinomas (2014)
  • Author(s): T. Sakashita, A. Homma, R. Hayashi, K. Kawabata, K. Yoshino, S. Iwae, Y. Hasegawa, K. Nibu, T. Kato, K. Shiga, K. Matsuura, N. Monden, M. Fuji
  • Journal Title: Oral Oncol. Volume: 40 Pages: 211-215
  • DOI: 10.1016/j.oraloncology.2014.03.003
  • NAID: 120005464719
  • Peer Reviewed
• [Journal Article] The potential diagnostic role of the number of ultrasonographic characteristics for patients with thyroid nodules evaluated as bethesdaI-v. (2014)
  • Author(s): Sakashita T, Homma A, Hatakeyama H, Mizumachi T, Kano S, Furusawa J, Iizuka S, Hoshino K, Hatanaka KC, Oba K, Fukuda S.
  • Journal Title: Frontiers in Oncology. Volume: 4 Pages: 261-261
  • DOI: 10.3389/fonc.2014.00261
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Salvage operations for patients with persistent or recurrent cancer of the maxillary sinus after superselective intra-arterial infusion of cisplatin with concurrent radiotherapy. (2014)
  • Author(s): Sakashita T, Homma A, Hatakeyama H, Kano S, Mizumachi T, Furusawa J, Yoshida D, Fujima N, Onimaru R, Tsuchiya K, Yasuda K, Shirato H, Suzuki F, Fukuda S.
  • Journal Title: Br J Oral Maxillofac Surg. Volume: 52 Pages: 323-328
  • DOI: 10.1016/j.bjoms.2014.01.018
  • NAID: 120005476154
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] The multi-institutional dose-finding trial of superselective intra-arterial infusion of cisplatin and concomitant radiotherapy for patients with locally advanced maxillary sinus cancer (JCOG1212, RADPLAT-MSC) (2016)
  • Author(s): Tomohiro Sakashita, Akihiro Homma, Rikiya Onimaru, Kazuto Matsuura, Hirotaka Shinomiya, Ryuichi Hayashi, Kiyoto Shiga, Hiroyuki Tachibana, Masato Fujii
  • Organizer: European society for medical oncology (ESMO) congress 2016
  • Place of Presentation: Copenhagen（Denmark）
  • Year and Date: 2016-10-07
  • Int'l Joint Research
• [Presentation] 舌癌N0症例における予防的頸部郭清術の役割と潜在的頸部転移陽性率に関する検討 (2016)
  • Author(s): 坂下智博、本間明宏、対馬那由多、福田諭
  • Organizer: 第29回口腔咽頭科学会
  • Place of Presentation: 松江テルサ（島根県松江市）
  • Year and Date: 2016-09-08
• [Presentation] The different nature of mucositis between cetuximab-based bioradiotherapy and platinum-based chemoradiotherapy in malignancies of the upper aerodigestive tracts. (2015)
  • Author(s): Tomohiro Sakashita, Akihiro Homma, Hiromitsu Hatakeyama, Satoshi Kano, Takatsugu Mizumachi, Tomohiko Kakizaki, Nayuta Tsushima, Takayoshi Suzuki, Rikiya Onimaru, Kazuhiko Tsuchiya, Koichi Yasuda, Hiroki Shirato, and Satoshi Fukuda
  • Organizer: 13th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery.
  • Place of Presentation: Hitotsubashi Hall,National Center of Science Building (Tokyo, Japan.)
  • Year and Date: 2015-12-03
  • Int'l Joint Research
• [Presentation] Acute toxicities associated with cetuximab-based bioradiotherapy and platinum-based chemoradiotherapy for head and neck squamous cell carcinomas. (2015)
  • Author(s): Tomohiro Sakashita, Akihiro Homma, Hiromitsu Hatakeyama, Satoshi Kano, Takatsugu Mizumachi, Tomohiko Kakizaki, Nayuta Tsushima, Takayoshi Suzuki, Rikiya Onimaru, Kazuhiko Tsuchiya, Koichi Yasuda, Hiroki Shirato, and Satoshi Fukuda
  • Organizer: 23rd Asia Pacific Cancer Conference
  • Place of Presentation: Grand Hyatt Hotel(Bali, Indonesia.)
  • Year and Date: 2015-08-20
  • Int'l Joint Research
• [Presentation] The role of cyclin D1 in surgical margins as a potential prognostic indicator. (2015)
  • Author(s): Tomohiro Sakashita, Nobuhiko Oridate, Akihiro Homma, Hiromitsu Hatakeyama, , Satoshi Kano, Takatsugu Mizumachi, Satoshi Fukuda.
  • Organizer: 4th Congress of Asian Society of Head and Neck Oncology/ 39th Annual Meeting of Japan Society for Head and Neck Cancer
  • Place of Presentation: Kobe International Conference Center(Kobe, Japan.)
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] 北海道大学における頭頸部癌治療の実際とセツキシマブ併用放射線治療の位置づけ (2014)
  • Author(s): 坂下智博
  • Organizer: 弘前頭頸部癌セミナー
  • Place of Presentation: 弘前大学医学部（青森県弘前市）
  • Year and Date: 2014-11-27
  • Invited
• [Presentation] 術前細胞診にて悪性が確定しなかった甲状腺結節におけるエコー陽性所見の数と悪性リスクの関係について (2014)
  • Author(s): 4.坂下智博、本間明宏、畠山博充、水町貴諭、加納里志、古沢純、飯塚さとし、干野季美子、畑中佳奈子、福田諭
  • Organizer: 第47回日本甲状腺外科学会
  • Place of Presentation: ヒルトン福岡シーホーク（福岡県福岡市）
  • Year and Date: 2014-10-30
• [Presentation] 頸部転移を有する中咽頭扁平上皮癌における初回頸部郭清術の意義 (2014)
  • Author(s): 3.坂下智博、本間明宏、林隆一、岩江信法、藤井正人
  • Organizer: 第27回日本口腔咽頭科学会総会
  • Place of Presentation: ホテルライフォート札幌（北海道札幌市）
  • Year and Date: 2014-09-11 – 2014-09-12
• [Presentation] 眼窩骨壁浸潤を伴う進行上顎洞癌における治療別生存率比較～眼窩内容温存全摘術の妥当性について～ (2014)
  • Author(s): 坂下智博、林隆一、本間明宏、松浦一登、加藤健吾、川端一嘉、松塚崇、岩江信法、大上研二、藤井正人
  • Organizer: 第38回日本頭頸部癌学会総会
  • Place of Presentation: 東京ファッションタウンビル（東京都江東区）
  • Year and Date: 2014-06-12 – 2014-06-13