Analysis of gene expression and function of TRP receptor in nasal polyps of eosinophilic chronic rhinosinusitis

• Project/Area Number: 26861360
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: University of Fukui
• Principal Investigator: Tokunaga Takahiro 福井大学, 医学部附属病院, 特命助教 (10464075)
• Co-Investigator(Renkei-kenkyūsha): FUJIEDA Shigeharu 福井大学, 医学部, 教授 (30238539) ; NOGUCHI Emiko 筑波大学, 医学医療系, 教授 (40344882)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 好酸球性副鼻腔炎 / 鼻茸 / TRPV3 / 次世代シーケンサー / トランスクリプトーム

Outline of Final Research Achievements

The number of patients with eosinophilic chronic rhinosinusitis (ECRS) tends to be increased in recent years in Japan. Whole transcriptome analysis with next generation sequencer (RNA-seq) was conducted to investigate molecular biological mechanism of ECRS. RNA-seq showed that 3 genes were significantly highly expressed in nasal polyps of ECRS. In these genes, TRPV3 (transient receptor potential cation channel, subfamily V, member 3) was the only gene which is not expressed in peripheral blood eosinophils.
Further study showed that TRPV3 was highly expressed in infiltrating eosinophils and mucosal epithelium of nasal polyps of ECRS. This results suggested that TRPV3 may play a role of refractoriness of ECRS.

Research Products

• [Journal Article] 好酸球性副鼻腔炎の診断と治療 (2016)
  • Author(s): 徳永 貴広, 藤枝 重治
  • Journal Title: 週刊日本医事新報 Volume: 4800 Pages: 36-41
  • NAID: 130003395285
• [Journal Article] Novel scoring system and algorithm for classifying chronic rhinosinusitis: The JESREC Study. (2015)
  • Author(s): Tokunaga T, Sakashita M, Haruna T, Asaka D, Takeno S, Ikeda H, Nakayama T, Seki N, Ito S, Murata J, Sakuma Y, Yoshida N, Terada T, Morikura I, Sakaida H, Kondo K, Yamada K, Okano M, Ohtori N, Yoshikawa M, et al
  • Journal Title: Allergy Volume: Epub ahead of print Issue: 8 Pages: 995-1003
  • DOI: 10.1111/all.12644
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 好酸球性副鼻腔炎 診断ガイドライン (JESREC Study) (2015)
  • Author(s): 藤枝 重治, 坂下 雅文, 徳永 貴広, 岡野 光博, 春名 威範, 他
  • Journal Title: 日本耳鼻咽喉科学会会報 Volume: 118 Pages: 728-735
  • NAID: 130005088297
• [Journal Article] Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin. (2015)
  • Author(s): Kanemaru K, Noguchi E, Tokunaga T, Nagai K, Hiroyama T, Nakamura Y, Tahara-Hanaoka S, Shibuya A.
  • Journal Title: PLoS One, 10(12), 2015 Volume: 10 Issue: 12 Pages: 1-16
  • DOI: 10.1371/journal.pone.0144436
  • NAID: 120007130029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 好酸球性副鼻腔炎の診断基準 (2015)
  • Author(s): 徳永貴広、坂下雅文、二之宮貴裕、意元義政、冨田かおり、森川太洋、藤枝重治
  • Journal Title: アレルギー・免疫 Volume: 22 Pages: 22-28
• [Presentation] Whole transcriptome解析(RNA-Seq)により同定された好酸球性副鼻腔炎関連遺伝子 (2016)
  • Author(s): 徳永 貴広, 意元 義政, 坂下 雅文, 高林 哲司, 藤枝 重治
  • Organizer: 第34回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 鳥羽国際ホテル
  • Year and Date: 2016-02-05
• [Presentation] Novel scoring system and algorithm for classifying eosinophilic chronic rhinosinusitis: The JESREC Study (2015)
  • Author(s): T.Tokunaga, M.Sakashita, T.Ninomiya, Y.Imoto, T.Morikawa, K.Tomita, T.Takabayashi, S.Fujieda & JESREC group
  • Organizer: The 9th Biennal Symposium of International Eosinophil Society
  • Place of Presentation: Holiday Inn Mart Plaza
  • Year and Date: 2015-07-16
  • Int'l Joint Research
• [Presentation] 次世代シーケンサーを用いたWhole transcriptome解析(RNA-seq)による好酸球性副鼻腔炎関連遺伝子の同定 (2015)
  • Author(s): 徳永 貴広, 二之宮 貴裕, 意元 義政, 坂下 雅文, 野口 恵美子, 藤枝 重治
  • Organizer: 第64回日本アレルギー学会学術大会
  • Place of Presentation: グランドプリンスホテル新高輪国際館パミール
  • Year and Date: 2015-05-27
• [Presentation] 次世代シーケンサーを用いたWhole transcriptome解析(RNA-seq)による好酸球性副鼻腔炎関連遺伝子の同定 (2015)
  • Author(s): 徳永貴広、二之宮貴裕、意元義政、坂下雅文、野口恵美子、藤枝重治
  • Organizer: 第64回日本アレルギー学会
  • Place of Presentation: 東京都・品川・グランドプリンスホテル新高輪 国際館パミール
  • Year and Date: 2015-05-26 – 2015-05-28
• [Presentation] 次世代シーケンサーを用いたWhole transcriptome解析(RNA-seq)による好酸球性副鼻腔炎関連遺伝子の同定 (2015)
  • Author(s): 徳永貴広、意元義政、坂下雅文、藤枝重治
  • Organizer: 第33回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 東京都・錦糸町・東武ホテルレバント東京
  • Year and Date: 2015-02-26 – 2015-02-28