Protection of spiral ganglion cells by adenoviral mediated overexpression of Tis21 in rodent.

• Project/Area Number: 26861395
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kumamoto University
• Principal Investigator: Momoko Ise 熊本大学, 医学部附属病院, 助教 (20573596)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Tis21遺伝子 / ラセン神経節細胞 / アデノウイルスベクター / 遺伝子導入による難聴治療 / らせん神経節 / Tis21 / 薬剤性難聴 / ラセン神経節 / マウスラセン神経節細胞 / シスプラチン / マウス

Outline of Final Research Achievements

Our research plan was obliged to modify by technical issues. First, we injected the adenoviral vector into the round window in guinea pig not mouse, because it was difficult for us to inject into the round window in mouse. Second, deafening was demonstrated by gentamicin and ethacrynic acid not cisplatin because of our laboratory environment.Auditory thresholds at one month after deafening were protected by adenoviral mediated overexpression of Tis21, but not at two weeks. The number of spiral ganglion cells in treated group at two weeks and one month after deafening was maintained compared to that in non-treated group.
Our results showed Tis21 had an important role to protect spiral ganglion cells against drug-induced hearing loss in guinea pig

Research Products

• [Journal Article] Neurogenesis of the spiral ganglion cells in the cochlea requires the transcriptional cofactor TIS21 (2015)
  • Author(s): Yamada T, Minoda R,Miwa T,Ise M,Takeda H,Yumoto E
  • Journal Title: Neurosci Lett Volume: 584 Pages: 265
  • DOI: 10.1016/j.neulet.2014.10.001
  • Peer Reviewed
• [Presentation] NEPA21（NEPAGENE社）を用いた耳胞への遺伝子導入の条件検討 (2015)
  • Author(s): 三輪徹
  • Organizer: 第25回日本耳科学会総会・学術講演会
  • Place of Presentation: 長崎(長崎ブリックホール)
  • Year and Date: 2015-10-08
• [Presentation] MPO-ANCA陽性を認めた難治性中耳炎の1症例 (2015)
  • Author(s): 伊勢桃子
  • Organizer: 第77回耳鼻咽喉科臨床学会総会・学術講演会
  • Place of Presentation: 静岡（アクトシティ浜松）
  • Year and Date: 2015-06-25
• [Presentation] Electroporation-Mediated Transuterine Gene Transfer Into Mouse Otocysts (EUGO) Utilizing NEPA21 Electoroporator (2015)
  • Author(s): Minoda R、Takeda H, Ise M, Yamada T, Yumoto E
  • Organizer: ARO 38th Annual Midwinter Meeting
  • Place of Presentation: 米国 Baltimore Marriott Waterfront
  • Year and Date: 2015-02-20 – 2015-02-27
• [Presentation] Protein transduction using arginine-rich cell penetrating peptides into the inner ear through the round window (2014)
  • Author(s): Takeda H, Minoda R, Yamada T, Ise M,Yumoto E
  • Organizer: Inner Ear Biology Workshop 2014
  • Place of Presentation: 京都 国立京都国際会館
  • Year and Date: 2014-11-01 – 2014-11-04