| Project/Area Number |
26861400
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Tfh細胞 / 脂質メディエーター / Breg細胞 / .濾胞ヘルパーT細胞 / 制御性B細胞 / 濾胞ヘルパーT細胞 / 遷移濾胞ヘルパーT細胞 |
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| Outline of Final Research Achievements |
The aim of this study was to determine the functional significance of Alox5-related lipid mediators during the processes of acquired humoral responses. The results of in vitro experiments using lymphocytes in tonsils and blood specimens showed that lipoxin A4 (LXA4) and, to a lesser extent, leukotriene B4 (LTB4) have the capacity to differentiate naïve CD4+ T cells into T follicular helper (Tfh) cells, which activate naïve B cells to form germinal centers. Such a function of LXA4 was further supported by results of in vitro studies using BML-111 and BOC-2, which are an agonist and antagonist to FPR2 of an LXA4-specific cell-surface receptor. Preferential expression of Alox5 in naïve B cells further lead to evidence indicating that Tfh cells can promote naïve B cells to secrete IL-10. Given that IL-10 stimulates and enhances the proliferation of B cells, such lipid mediators would have a potential role in the presentation of integrities of lymphoid follicles.
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