Generation of tracheal chondrocytes from human iPS cells
| Project/Area Number |
26861405
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
Yoshie Susumu 福島県立医科大学, 医学部, 助教 (70705459)
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| Research Collaborator |
OMORI Koichi
HAZAMA Akihiro
WADA Ikuo
IMAIZUMI Mitsuyoshi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ヒトiPS細胞 / 気管 / 軟骨 / 再生 |
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| Outline of Final Research Achievements |
The aim of this study is to regenerate tracheal defect by the use of an artificial trachea with human iPS cell-derived tracheal chondrocytes as a frame and collagen sponge as a scaffold. First, we could successfully induce human iPS cells into paraxial mesoderm with high efficiency using low molecular weight compounds and growth factors. Next, tracheal chondrocytes could be generated from human iPS cell-derived paraxial mesoderm by the overexpression of transcriptional factor A, which is related to chondrocyte differentiation. Finally, an artificial trachea with human iPS cell-derived tracheal chondrocytes and collagen sponge was implanted into tracheal defects in nude rats. At 14 d after implantation, the survaival of rat was confirmed. These results raised the possibility that our artificial trachea can regenerate tracheal defect.
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Report
(4 results)
Research Products
(3 results)
