| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
The aim of this study is to regenerate tracheal defect by the use of an artificial trachea with human iPS cell-derived tracheal chondrocytes as a frame and collagen sponge as a scaffold. First, we could successfully induce human iPS cells into paraxial mesoderm with high efficiency using low molecular weight compounds and growth factors. Next, tracheal chondrocytes could be generated from human iPS cell-derived paraxial mesoderm by the overexpression of transcriptional factor A, which is related to chondrocyte differentiation. Finally, an artificial trachea with human iPS cell-derived tracheal chondrocytes and collagen sponge was implanted into tracheal defects in nude rats. At 14 d after implantation, the survaival of rat was confirmed. These results raised the possibility that our artificial trachea can regenerate tracheal defect.
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