Examination of the infuluence of zinc on the expression of taste receptor genes(T1Rs) in the rat tongue

Research Project

Project/Area Number	26861422
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Otorhinolaryngology
Research Institution	Nihon University
Principal Investigator	TANAKA Makoto 日本大学, 医学部, 助教 (00526121)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	味覚受容体遺伝子 / TAS1Rｓ / 味覚受容体 / 亜鉛
Outline of Final Research Achievements	We evaluated the effects of zinc deficiency on gene expression for T1Rs, sweet and umami taste receptors, using rat circumvallate papilla epithelium by RT-PCR.Zinc deficiency did not affect gene expression for T1R1,T1R2,T1R3. In situ hybridization was performed to investigate the expression and localization of T1Rs. The expression of T1Rs was specifically found in taste cells in taste bud.