Development of a novel treatment for inner ear deafness -inhibition of the expression of Cochlin with RNAi-
| Project/Area Number |
26861423
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
Sekine Kuwon 日本医科大学, 医学部, 助教 (20566377)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUMURA Tomohiro 日本医科大学, 医学部, 助教 (20297930)
IKEZONO Tetsuo 埼玉医科大学, 医学部, 教授 (80277491)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | inner ear / miRNA / COCH / cochlin / Cochlin / mRNA |
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| Outline of Final Research Achievements |
This study was initiated to check whether the onset of hearing loss can be prevented by inhibiting the expression of the causative protein of DNFA9 in the adult onset-type hereditary hearing loss. First, it is necessary to obtain cells, which are stably expressing Cochlin. However, since there are no cells in the commercial-use cell lines expressing Cochlin, isolated fibroblasts from the inner ear of mice and immortalized cell lines were used to attempt forced expression of COCH mRNA using an expression vector - but cells which stably express Cochlin were unable to be obtained. In addition, as a preliminary experiment, miRNA that could inhibit Cochlin was searched for in miRNA in the human inner ear but was not confirmed.
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Report
(5 results)
Research Products
(1 results)
