Possible role of CD271 for maintaining stemness and application to treatment in HPC.

• Project/Area Number: 26861427
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Miyagi Prefectural Hospital Organization Miyagi Cancer Center
• Principal Investigator: Mochizuki Mai 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), 発がん制御研究部, 研究技師 (40726303)
• Research Collaborator: Tamai Keiichi ; Imai Takayuki ; Sugawara Sayuri
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 下咽頭癌 / CD271 / 細胞周期 / 頭頸部癌 / 細胞増殖 / 浸潤 / がん幹細胞

Outline of Final Research Achievements

We previously reported that CD271 (p75 neurotrophin receptor) is a marker for tumor formation and correlated with a poor prognosis in human hypopharyngeal cancer. To elucidate the role of CD271 in HPC, we established HPC cell lines and CD271-knockdown cells using siRNA. We revealed that CD271-knockdown strongly suppressed the tumorigenesis and cell growth.In addition, CD271 depletion diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity and by accelerating the migration signaling pathway in HPC.

Research Products

• [Journal Article] Xenotransplantation elicits salient tumorigenicity of adult T-cell leukemia-derived cells via aberrant AKT activation. (2016)
  • Author(s): Yamaguchi K, Takanashi T, Nasu K, Tamai K, Mochizuki M, Satoh I, Ine S, Sasaki O, Satoh K, Tanaka N, Harigae H, Sugamura K.
  • Journal Title: Cancer Sci. Volume: ePub Issue: 5 Pages: 638-643
  • DOI: 10.1111/cas.12921
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Suppressive expression of CD274 increases tumorigenesis and cancer stem cell phenotypes in cholangiocarcinoma (2014)
  • Author(s): Keiichi Tamai, Mao Nakamura, Masamichi Mizuma, Mai Mochizuki, Misa Yokoyama, Hiroyuki Endo, Kazunori Yamaguchi, Takayuki Nakagawa, Masaaki Shiina, Michiaki Unno, Koji Muramoto, Ikuro Sato, Kennichi Satoh, Kazuo Sugamura, and Nobuyuki Tanaka
  • Journal Title: Cancer Science Volume: 105 Issue: 6 Pages: 667-674
  • DOI: 10.1111/cas.12406
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 下咽頭癌においてCD271は増殖能・浸潤能を制御する (2016)
  • Author(s): 望月 麻衣、玉井 恵一、今井 隆之、中村 真央、小鎌 直子、本橋 ほづみ、菅村 和夫
  • Organizer: 平成27年度「がん研究分野の特性等を踏まえた支援活動」公開シンポジウム
  • Place of Presentation: 一橋講堂
  • Year and Date: 2016-02-09
• [Presentation] 頭頸部癌におけるCD271の機能性 (2015)
  • Author(s): 望月麻衣、今井隆之、松浦一登、小鎌直子、玉井恵一、本橋ほづみ、菅村和夫、田中伸幸
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] CD271 marks cancer initiating cells in hypopharyngeal carcinoma with enhanced proliferative potential (2015)
  • Author(s): Mai Mochizuki, Takayuki Imai, Keiichi Tamai, Hozumi Motohashi, Nobuyuki Tanaka
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-10-08
• [Presentation] 頭頸部がん幹細胞における CD271 の役割の解明 (2015)
  • Author(s): 望月麻衣、今井隆之、松浦一登、小鎌直子、玉井恵一、本橋ほづみ、菅村和夫、田中伸幸
  • Organizer: 日本生化学会東北支部第 81 回例会・シンポジウム
  • Place of Presentation: さくらホール
  • Year and Date: 2015-05-09
• [Presentation] CD271 plays critical roles in invading cancer cells in hypopharyngeal carcinoma. (2014)
  • Author(s): Mai Mochizuki1, Imai Takayuki, Nobuyuki Tanaka et al.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27