| Project/Area Number |
26861432
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Tohoku University (2015)
Iwate University (2014) |
|---|
Principal Investigator |
Murayama Namie 東北大学, 大学病院, 産学官連携研究員 (60597516)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 眼生理 / パッチクランプ |
|---|
| Outline of Final Research Achievements |
Channelrhodopsin, a molecular of light gated ion channel, is a promising therapeutic tool for vision restoration. Nonetheless, there are still few studies on the channelrhodopsin-mediated retinal neurotransmission. The purpose in this study is to investigate the response of the individual cell on the retinal slice by a patch clamp recordings.
We set up the method of the slice preparation and the slice patch clamp system. We made a construction of an adeno-associated virus vector (AAV-mGluR6-ChR2V) which specifically the target gene transduced into On-bipolar cells and could observe the gene expression in the On-bipolar cells on the cryo-sections. However, the transduction efficiencies into the On-bipolar cells was quite low, and it was difficult to find ChR2-expressing cells on the slice patch clamp recordings. We will try the patch clamp recordings on the retinal whole mount specimen.
|
