| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Age-related macular degeneration and proliferative vitreoretinopathy are the major cause of vision loss and poor visual prognosis due to the chorioretinal fibrosis. The epithelial mesenchymal transition of retinal pigment epithelium (RPE) has been theorized to play a critical role in the development of such pathological fibrosis. The purpose of this study was to elucidate the change of the molecular network in the fibrotic change of the human RPE cell line (ARPE-19) and to investigate if the fibrotic change of ARPE-19 could be inhibited by HDAC inhibitor. The morphology of the ARPE-19 cultured with TGFβ2 or TNFα was changed to the spindle shape. The expression of αSMA, CD44, and MMP9 were significantly increased by TGFβ2+TNFα. The morphological change of ARPE-19 cultured in the medium with TGFβ2 or TNFα was suppressed by a HDAC inhibitor.
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