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The analysis of the phase transition of retinal pigment epithelium and the epigenetic regulation in age-related macular degeneration

Research Project

Project/Area Number 26861464
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Hatanaka Hiroki  京都府立医科大学, 医学(系)研究科(研究院), 助教 (80368050)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords組織線維化 / エピジェネティック / 眼内増殖性疾患 / 加齢黄斑変性 / 網膜色素上皮 / 線維化 / 線維性変化
Outline of Final Research Achievements

Age-related macular degeneration and proliferative vitreoretinopathy are the major cause of vision loss and poor visual prognosis due to the chorioretinal fibrosis. The epithelial mesenchymal transition of retinal pigment epithelium (RPE) has been theorized to play a critical role in the development of such pathological fibrosis. The purpose of this study was to elucidate the change of the molecular network in the fibrotic change of the human RPE cell line (ARPE-19) and to investigate if the fibrotic change of ARPE-19 could be inhibited by HDAC inhibitor. The morphology of the ARPE-19 cultured with TGFβ2 or TNFα was changed to the spindle shape. The expression of αSMA, CD44, and MMP9 were significantly increased by TGFβ2+TNFα. The morphological change of ARPE-19 cultured in the medium with TGFβ2 or TNFα was suppressed by a HDAC inhibitor.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2016

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Effect of Epigenetic Regulation on Fibrotic Change in Retinal Pigment Epithelium Cells2016

    • Author(s)
      畑中宏樹、向敦史、米田一仁、山田潤、外園千恵、木下茂、羽室淳爾
    • Organizer
      ARVO 2016
    • Place of Presentation
      シアトル
    • Year and Date
      2016-05-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 網膜色素上皮の線維性変化に対するエピジェネティックな調整機構の影響2016

    • Author(s)
      畑中宏樹、向敦史、米田一仁、山田潤、外園千恵、木下茂、羽室淳爾
    • Organizer
      第120回日本眼科学会総会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-04-07
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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