| Project/Area Number |
26861469
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Juntendo University |
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Principal Investigator |
Ouchi Ayumi 順天堂大学, 医学部, 助教 (80645664)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 増殖糖尿病網膜症 / 増殖硝子体網膜症 / マイクロRNA / 上皮間葉系移行 / 糖尿病網膜症 / microRNA / microRNA-21 / 網膜色素上皮細胞 / 硝子体 |
|---|
| Outline of Final Research Achievements |
We focused on microRNAs that are expected as targets of a novel drug with the aim of elucidating the fibroproliferative pathology of proliferative diabetic retinopathy (PDR) or proliferative vitreoretinopathy, which is a main cause of blindness. MicroRNA-21 (miR-21) increased in vitreous of PDR, and its expression was elevated in a fibroproliferative pathology-specific manner. In retinal pigment epithelial cell line, the expression of miR-21 was induced in the epithelial-mesenchymal transition process, which is also important for retinal fibroproliferation, and its expression elevation was promoted under diabetic conditions. On functional analysis, it was revealed that miR-21 promote the proliferation and the migration of retinal pigmented epithelial cells. From these results, we found a new possibility that miR-21 is involved in the pathogenesis of fibroproliferation in PDR.
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