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Development of non-invasive diagnostic tools for childhood cancers using digital PCR

Research Project

Project/Area Number 26861483
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pediatric surgery
Research InstitutionHiroshima University

Principal Investigator

KURIHARA SHO  広島大学, 大学病院, 医科診療医 (40724894)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords癌 / 遺伝子 / 小児
Outline of Final Research Achievements

MYCN amplification was detected by digital PCR using of plasma DNA and bone marrow DNA samples derived from 23 neuroblastoma cases before treatment but became undetectable after surgery except for 6 cases with metastasis. ALK mutations in 2 neuroblastoma cases and CTNNB1 mutations in 3 hepatoblastoma cases were detected in plasma DNA at diagnosis but became undetectable more than 5 days after surgery. The library for 12 kinds of chimeric DNAs in small round cell tumors was designed. By the next generation sequencing using this library, chimeric genes were detected in 1 Ewing family tumor and 2 rhabdomyosarcomas but became undetecble after surgery. Plasma exosomes were concentrated and then microRNA was detected in these exosome samples in 4 cases.
These data suggested that digital PCR methods using blood sample is useful for diagnosis of childhood cancers.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (14 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Circulating free DNA as non-invasive diagnostic biomarker for childhood solid tumors2015

    • Author(s)
      Kurihara S, Ueda Y, Onitake Y, Sueda T, Ohta E, Morihara N, Hirano S, Irisuna F, Hiyama E
    • Journal Title

      Journal of Pediatric Surgery

      Volume: 50 Issue: 12 Pages: 2094-2097

    • DOI

      10.1016/j.jpedsurg.2015.08.033

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mortality and morbidity in primarily resected hepatoblastomas in Japan: Experience of the JPLT (Japanese Study Group for Pediatric Liver Tumor) trials2015

    • Author(s)
      Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y
    • Journal Title

      Journal of Pediatric Surgery

      Volume: 50 Issue: 12 Pages: 2098

    • DOI

      10.1016/j.jpedsurg.2015.08.035

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 肝芽腫の分子生物学2015

    • Author(s)
      檜山英三, 上田祐華, 栗原 将
    • Journal Title

      小児外科

      Volume: 47 Pages: 181-188

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical features of ATRX or DAXX mutated neuroblastoma2014

    • Author(s)
      Kurihara S, Hiyama E, Onitake Y, Yamaoka E, Hiyama K
    • Journal Title

      Journal of Pediatric Surgery

      Volume: 49 Issue: 12 Pages: 1835-1838

    • DOI

      10.1016/j.jpedsurg.2014.09.029

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pediatric Hepatoblastoma: diagnosis and treatment2014

    • Author(s)
      Hiyama E
    • Journal Title

      Translational Pediatrics

      Volume: 3 Pages: 293-299

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 門脈体循環シャントにて経過観察している3症例2015

    • Author(s)
      栗原 将, 檜山 英三, 上田 祐華
    • Organizer
      第31回日本小児外科学会秋季シンポジウム
    • Place of Presentation
      熊本市
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] Prediction of prognosis by preoperative chemotherapy response in hepatoblastoma patients treated by JPLT-2 protocol2015

    • Author(s)
      Hiyama E, Hishiki T, Ida K, Watanabe K, Oue T, Yano M, Hoshino K, Iehara T, Koh K, Tanaka Y, Kurihara S, Ueda Y
    • Organizer
      SIOP 2015
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Integrated exome analysis in childhood hepatoblastoma: Biological approach for molecular targeting2015

    • Author(s)
      Hiyama E, Kurihara S, Onitake Y, Ueda Y, Morihara N, Fukuba I, Komatsu R
    • Organizer
      SIOP 2015
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] AFP高値を認めた肝限局性過形成の1症例2015

    • Author(s)
      栗原 将
    • Organizer
      第54回日本小児外科学会中国四国地方会
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 門脈-右房シャントを呈した静脈管欠損症の1例2015

    • Author(s)
      栗原 將, 檜山 英三, 鬼武 美幸
    • Organizer
      第52回日本小児外科学会学術集会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Circulating free DNA as non-invasive diagnostic biomarker for childhood solid tumors2015

    • Author(s)
      Kurihara S, Ueda Y, Onitake Y, Sueda T, Ohta E, Morihara N, Hirano S, Irisuna F, Hiyama E
    • Organizer
      48th Annual Meeting of the Pacific Association of Pediatric Surgeons
    • Place of Presentation
      Jeju, South Kore
    • Year and Date
      2015-05-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mortality and morbidity in primarily resected hepatoblastomas in Japan: experience of JPLT (Japanese study group for pediatric liver tumor) trials2015

    • Author(s)
      Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y
    • Organizer
      48th Annual Meeting of the Pacific Association of Pediatric Surgeons
    • Place of Presentation
      Jeju, South Kore
    • Year and Date
      2015-05-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 肺転移を有した肝芽腫6例の外科的検討2014

    • Author(s)
      栗原 将, 鬼武美幸, 三木瑞香, 中村和洋, 小林正夫, 檜山英三
    • Organizer
      第56回日本小児血液・がん学会学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2014-11-28 – 2014-11-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Outcome and morbidity of primary resection of hepatoblastoma in JPLT-1 and 2 protocols2014

    • Author(s)
      Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S
    • Organizer
      SIOP2014
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2014-10-22 – 2014-10-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 小児固形腫瘍における循環遊離DNAと循環がん細胞のがん診断への応用2014

    • Author(s)
      檜山英三, 檜山桂子, 林 陽子, 森原なぎさ, 平野尚子, 福場郁子, 山岡絵美, 栗原 将
    • Organizer
      第73回日本癌学会学術集会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] urgical managements for refractory neuroblastoma in young children2014

    • Author(s)
      Hiyama E, Kurihara S, Onitake Y, Ueda Y, Miki M, Kawaguchi H, Nakamura K, Kobayashi M
    • Organizer
      BAPS61st Annual Congress
    • Place of Presentation
      Edinburgh, Scotland
    • Year and Date
      2014-07-22 – 2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Central venous catheter-related complications in children with malignancy2014

    • Author(s)
      Kurihara S, Hiyama E, Onitake Y, Miki M, Nakamura K, Kawaguchi H, Kobayashi M
    • Organizer
      PAPS2014
    • Place of Presentation
      Calgary, Canada
    • Year and Date
      2014-05-25 – 2014-05-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Clinical feature of ATRX or DAXX mutated neuroblastoma2014

    • Author(s)
      Hiyama E, Kurihara S, Onitake Y, Yamaoka E, Fukuba I, Hiyama K
    • Organizer
      PAPS2014
    • Place of Presentation
      Calgary, Canada
    • Year and Date
      2014-05-25 – 2014-05-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 肝芽腫の縦隔再発に対してICG蛍光法を使用した経験2014

    • Author(s)
      栗原 将, 鬼武美幸, 小倉 薫, 檜山英三
    • Organizer
      第55回日本中国四国小児がん研究会
    • Place of Presentation
      広島県福山市
    • Year and Date
      2014-04-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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