Development of non-invasive diagnostic tools for childhood cancers using digital PCR
Project/Area Number |
26861483
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pediatric surgery
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Research Institution | Hiroshima University |
Principal Investigator |
KURIHARA SHO 広島大学, 大学病院, 医科診療医 (40724894)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 癌 / 遺伝子 / 小児 |
Outline of Final Research Achievements |
MYCN amplification was detected by digital PCR using of plasma DNA and bone marrow DNA samples derived from 23 neuroblastoma cases before treatment but became undetectable after surgery except for 6 cases with metastasis. ALK mutations in 2 neuroblastoma cases and CTNNB1 mutations in 3 hepatoblastoma cases were detected in plasma DNA at diagnosis but became undetectable more than 5 days after surgery. The library for 12 kinds of chimeric DNAs in small round cell tumors was designed. By the next generation sequencing using this library, chimeric genes were detected in 1 Ewing family tumor and 2 rhabdomyosarcomas but became undetecble after surgery. Plasma exosomes were concentrated and then microRNA was detected in these exosome samples in 4 cases. These data suggested that digital PCR methods using blood sample is useful for diagnosis of childhood cancers.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Mortality and morbidity in primarily resected hepatoblastomas in Japan: Experience of the JPLT (Japanese Study Group for Pediatric Liver Tumor) trials2015
Author(s)
Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y
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Journal Title
Journal of Pediatric Surgery
Volume: 50
Issue: 12
Pages: 2098-2101
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Prediction of prognosis by preoperative chemotherapy response in hepatoblastoma patients treated by JPLT-2 protocol2015
Author(s)
Hiyama E, Hishiki T, Ida K, Watanabe K, Oue T, Yano M, Hoshino K, Iehara T, Koh K, Tanaka Y, Kurihara S, Ueda Y
Organizer
SIOP 2015
Place of Presentation
Cape Town, South Africa
Year and Date
2015-10-08
Related Report
Int'l Joint Research
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[Presentation] Mortality and morbidity in primarily resected hepatoblastomas in Japan: experience of JPLT (Japanese study group for pediatric liver tumor) trials2015
Author(s)
Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y
Organizer
48th Annual Meeting of the Pacific Association of Pediatric Surgeons
Place of Presentation
Jeju, South Kore
Year and Date
2015-05-17
Related Report
Int'l Joint Research
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[Presentation] 肺転移を有した肝芽腫6例の外科的検討2014
Author(s)
栗原 将, 鬼武美幸, 三木瑞香, 中村和洋, 小林正夫, 檜山英三
Organizer
第56回日本小児血液・がん学会学術集会
Place of Presentation
岡山市
Year and Date
2014-11-28 – 2014-11-30
Related Report
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[Presentation] Outcome and morbidity of primary resection of hepatoblastoma in JPLT-1 and 2 protocols2014
Author(s)
Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S
Organizer
SIOP2014
Place of Presentation
Toronto, Canada
Year and Date
2014-10-22 – 2014-10-25
Related Report
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