Application to the wound healing of the human skin derived precursor cells

• Project/Area Number: 26861498
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Kyoto University
• Principal Investigator: Yoshikawa Katsuhiro 京都大学, 医学(系)研究科(研究院), 助教 (10583156)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 真皮幹細胞 / SKP / 皮膚再生 / 高齢者 / 高齢者皮膚

Outline of Final Research Achievements

Skin derived precursor cells (sphere) were collected from human skin samples in suspension culture. The cells were passaged and proliferated using growth medium in adherent culture, then turned into re-sphere in suspension culture. The pluripotency of the cells were confirmed by neural, adipogenic and osteogenic differentiation assay. Expression of stem cell markers was confirmed in the mRNA.In the transplantation experiment of the cells that were cultured on collagen sponge to nude mice, the trend of differentiation to the sebaceous gland that are appendages of the skin was observed. When compared to the fibroblasts, thicker dermis-like tissue was formed.

Research Products

• [Journal Article] A Case of Extensive Sacral Decubitus Ulcer Complicated by an Epidural Abscess (2016)
  • Author(s): Murata, Mai MD; Yoshikawa, Katsuhiro MD, PhD; Hiratsuka, Munehisa MD; Suzuki, Shigehiko MD, PhD
  • Journal Title: PRS Global Open Volume: -
  • Peer Reviewed / Open Access
• [Presentation] 術前にNPWTを併用する褥瘡手術 (2016)
  • Author(s): 吉川 勝宇、江野尻竜樹、坂本道治、齋藤晋、河合勝也、鈴木茂彦
  • Organizer: 第21回日本形成外科手術手技学会
  • Place of Presentation: 大宮
  • Year and Date: 2016-02-13
• [Presentation] 当院でのDIEP-flapによる乳房再建の実際 (2014)
  • Author(s): 吉川勝宇, 江野尻竜樹, 山脇聖子, 鈴木茂彦
  • Organizer: 第41回日本マイクロサージャリ―学会学術集会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] オンコプラスティックサージェリーについて (2014)
  • Author(s): 吉川勝宇
  • Organizer: 第20回京都乳癌コンセンサス会議
  • Place of Presentation: ウエステイン都ホテル
  • Year and Date: 2014-06-28
  • Invited
• [Presentation] 当科における胸腔胸壁再建術について (2014)
  • Author(s): 吉川勝宇, 上田真帆,中村陽子, 齊藤晋, 益岡弘, 山脇聖子, 内藤素子, 河合勝也, 鈴木茂彦
  • Organizer: 第57回 日本形成外科学会総会・学術集会
  • Place of Presentation: 長崎ブリックホール
  • Year and Date: 2014-04-09