Development and application of bioimaging technology to contribute to the spatiotemporal and functional analysis in inflammatory miRNA

• Project/Area Number: 26861503
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Nagasaki University
• Principal Investigator: TANAKA Katsuya 長崎大学, 病院(医学系), 医員 (70722750)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: miRNA / 炎症 / イメージング / 皮膚創傷治癒 / 炎症細胞

Outline of Final Research Achievements

This study has been performed to elucidate the organizational and molecular correlation influencing on tissue repair mechanism including scar formation by spatiotemporal dynamics of inflammatory cells at skin wound. And we achieved to identify the skin wound inflammation-related microRNA (miRNA) which was noted having various functions. Furthermore, we produced the miRNA knockout mice and revealed that the one of inflammation-related miRNA affected the phagocytosis and infection control function of neutrophils having a central role in innate immunity by using bioimaging technique. The imaging techniques used in this study that employed a confocal fluorescent microscope can be a technique that is widely used in the world.

Research Products

• [Presentation] 創傷治癒における miR-142 の機能解析 (2015)
  • Author(s): 田中 克弥
  • Organizer: 第27回長崎障害者支援再生医療研究会
  • Place of Presentation: 長崎大学医学部 良順会館（長崎）
  • Year and Date: 2015-12-08
• [Presentation] miR-142 は皮膚創部における黄色ブドウ球菌感染防御に必須である (2015)
  • Author(s): 田中 克弥
  • Organizer: 第45回日本創傷治癒学会
  • Place of Presentation: JPタワーホール＆カンファレンス（東京）
  • Year and Date: 2015-11-30
• [Presentation] Functions of miR-142 in skin wound healing: regulation of the hematopoiesis system and tissue repair (2015)
  • Author(s): Katsuya Tanaka
  • Organizer: Gordon Research Conferences, Tissue repair & regeneration
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 炎症関連microRNAのマウス皮膚創傷治癒における病態生理学的役割 (2014)
  • Author(s): 田中 克弥
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Solitary fibrous tumorの3例 (2014)
  • Author(s): 田中 克弥
  • Organizer: 第32回日本頭蓋顎顔面外科学会学術集会
  • Place of Presentation: 大阪中央公会堂(大阪)
  • Year and Date: 2014-11-06 – 2014-11-07
• [Presentation] Pathophysiological role of inflammation-related microRNA in murine skin wound healing (2014)
  • Author(s): 田中 克弥
  • Organizer: EMBO Conference ‘The Molecular & Cellular Basis of Regeneration & Tissue Repair’
  • Place of Presentation: Sant Feliu de Guixols(spain）
  • Year and Date: 2014-09-06 – 2014-09-10
• [Presentation] 非固着性コンタクトレイヤーの創傷における有用性～臨床研究を通じて～ (2014)
  • Author(s): 田中 克弥
  • Organizer: 第57回日本形成外科学会総会・学術集会
  • Place of Presentation: 長崎ブリックホール(長崎)
  • Year and Date: 2014-04-09 – 2014-04-11