| Project/Area Number |
26861520
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Mie University |
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Principal Investigator |
Kawamoto Eiji 三重大学, 医学部附属病院, 助教 (20577415)
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| Research Collaborator |
OKAMOTO Takayuki 三重大学, 大学院医学系研究科 分子病態学, 助教 (30378286)
IMAI Hiroshi 三重大学, 医学部附属病院 救命救急センター, 教授 (00184804)
SHIMAOKA Motomu 三重大学, 大学院医学系研究科 分子病態学, 教授 (40281133)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Thrombomodulin / LFA-1 integrin / Mac-1 integrin / トロンボモジュリン / LFA-1インテグリン / Mac-1インテグリン / セリンスレオニンリッチドメイン / ICAM-1 / インテグリン / 凝固と炎症のクロストーク |
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| Outline of Final Research Achievements |
LFA-1 and Mac-1 integrins regulate leukocyte trafficking in health and disease by binding primarily to IgSF ligand ICAM-1 and ICAM-2 on endothelial cells. Here we have shown that the anti-coagulant molecule thrombomodulin (TM), found on the surface of endothelial cells, functions as a potentially new ligand for leukocyte integrins. Furthermore, we show that the serine/threonine-rich domain of TM is required for the interaction with the LFA-1 and Mac-1 integrins to occur on PBMCs. These results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to TM, thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. In fact, integrin-TM interactions might be involved in the dynamic regulation of leukocyte adhesion with endothelial cells.
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