| Project/Area Number |
26861547
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
FUJII SHINSUKE 大阪大学, 医学(系)研究科(研究院), 特任助教(常勤) (60452786)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 唾液腺 / Wnt / 増殖 / 分化 / マウス / 発生 / Wntシグナル |
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| Outline of Final Research Achievements |
In this study, it was shown how Wnt signaling controls epithelial morphogenetic changes, such as growth and branching, and differentiation using the salivary gland as a model. When mesenchyme-free submandibular salivary gland (SMG) epithelium at E13 was cultured with IWP2, a Wnt secretion inhibitor, growth and branching of SMG were suppressed. Activation of Wnt/β-catenin signaling promoted proliferation of SMG duct progenitor cells which were positive for Ki67 staining. Experiments using genetically manipulated mice (Ctnnb1(Ex3)Δ/Δ ) revealed that the numbers of multiple acinar-like structures, defined by the existence of a central lumen, in Ctnnb1(Ex3)Δ/Δ mice were reduced compared with those of wild type mice. And protein levels of aquaporin 5, parotid secretory protein and phosphorylated AKT in Ctnnb1(Ex3)Δ/Δ mice were suppressed.
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