A Novel Mechanism of Osteo-network through MicroRNAs

• Project/Area Number: 26861548
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Takei Yuichiro 広島大学, 医歯薬保健学研究院(歯), 助教 (10711377)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マイクロRNA / 骨基質小胞 / 破骨細胞形成

Outline of Final Research Achievements

The balance between bone formation and bone destruction is fundamental on the skeletal homeostasis.
We found the microRNAs (miRNAs) included in bone matrix vesicles (BMVs), that is specifically derived from bone-forming osteoblasts. Furthermore, it had been uncovered osteoblasts applied BMV as a signal transducer and suppressed the differentiation from macrophages into osteoclasts, bone-absorbing cells. Especially, it was suggested that a certain miRNA, miR-125b involved in BMVs was crucial in this pathway.
This study contributes a discovery of a novel mechanism of bone metabolism.

Research Products

• [Journal Article] Bone-derived miRNA as a mediator of cell-cell communication. (2015)
  • Author(s): Takei Y, Minamizaki T, Yoshioka H, Yoshiko Y.
  • Journal Title: Proceedings of 6th Hiroshima Conference on Education and Science in Dentistry Volume: 6 Pages: 71-72
• [Journal Article] Functional diversity of fibroblast growth factors in bone formation. (2015)
  • Author(s): Takei Y, Minamizaki T, Yoshiko Y.
  • Journal Title: Int J Endocrinol. Volume: 2015 Pages: 729352-729352
  • DOI: 10.1155/2015/729352
  • NAID: 120006824054
  • Peer Reviewed / Open Access
• [Journal Article] 骨・血管相関とmicroRNA (2014)
  • Author(s): 竹井悠一郎、吉子裕二
  • Journal Title: 臨床化学 Volume: 43 Pages: 106-111
• [Presentation] Bone-derived miRNA as a mediator of cell-cell communication. (2015)
  • Author(s): Takei Y, Minamizaki T et al.
  • Organizer: 6th Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2015-10-23
  • Int'l Joint Research / Invited
• [Presentation] Matrix Vesicles Mediate the Cell-to-Cell Transmission of MicroRNA-125b as an Inhibitor of Osteoclastic Bone Resorption. (2015)
  • Author(s): Takei Y, Nakao Y et al.
  • Organizer: The American Society for Bone and Mineral Research
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] MicroRNAs Involved in Bone Metabolism Are Transported into Matrix Vesicles during Bone Formation. (2015)
  • Author(s): Nakao Y, Takei Y et al.
  • Organizer: The American Society for Bone and Mineral Research
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] 破骨細胞形成および／または機能抑制剤、破骨細胞形成および／または機能促進剤並びに破骨細胞形成および／または機能を抑制または促進する薬剤のスクリーニング法 (2015)
  • Inventor(s): 吉子裕二、竹井悠一郎、南崎明子、吉岡広陽、越智保夫(小野薬品)
  • Industrial Property Rights Holder: 広島大学、小野薬品工業株式会社
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-162851
  • Filing Date: 2015-08-20