Oral bacterial species having nitrate reducing activity

• Project/Area Number: 26861552
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: NAMBU Takayuki 大阪歯科大学, 歯学部, 講師 (80367903)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 口腔細菌学 / 硝酸還元 / 口腔細菌

Outline of Final Research Achievements

There is a diverse microbiota in the oral cavity, in which specific oral bacteria are able to reduce nitrate to nitrite. We have determined complete genome sequences of four strains including nitrate reducing Actinomyces and Rothia species. Additionally, through the development a novel selective medium for isolating oral Rothia species, we compared the nitrate reducing activities of Rothia species.

Research Products

• [Journal Article] Genome Sequence of Actinomyces naeslundii Strain ATCC 27039, Isolated from an Abdominal Wound Abscess. (2016)
  • Author(s): Mashimo C, Yamane K, Yamanaka T, Maruyama H, Wang PL, Komasa S, Okazaki J, Nambu T.
  • Journal Title: Genome Announc Volume: 4(6) Issue: 6
  • DOI: 10.1128/genomea.01443-16
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Complete Genome Sequence of Rothia aeria Type Strain JCM 11412, Isolated from Air in the Russian Space Laboratory Mir. (2016)
  • Author(s): Nambu T, Tsuzukibashi O, Uchibori S, Yamane K, Yamanaka T, Maruyama H, Wang PL, Mugita N, Morioka H, Takahashi K, Komasa Y, Mashimo C.
  • Journal Title: Genome Announcements Volume: 4 Issue: 6
  • DOI: 10.1128/genomea.01444-16
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Complete genome sequence of Rothia mucilaginosa strain NUM-Rm6536, isolated from a human oral cavity. (2015)
  • Author(s): Nambu T, Tsuzukibashi O, Uchibori S, Mashimo C.
  • Journal Title: Genome Announcements Volume: 3 Issue: 5
  • DOI: 10.1128/genomea.01122-15
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Complete Genome Sequence of Prevotella intermedia Strain 17-2 (2015)
  • Author(s): Nambu T, Yamane K, Maruyama H, Mashimo C, Yamanaka T.
  • Journal Title: Genome Announc Volume: 3 Issue: 4
  • DOI: 10.1128/genomea.00951-15
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Variations of nitrate-reducing activity in oral Rothia mucilaginosa. (2015)
  • Author(s): Mashimo C, Tsuzukibashi O, Uchibori S, Nambu T.
  • Journal Title: Journal of Oral Tissue Engineering Volume: 13 Pages: 18-26
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 形質転換可能なRothia属菌株の分離とゲノム解読 (2015)
  • Author(s): 南部隆之，真下千穂
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-12
• [Presentation] 口腔Rothia属細菌における硝酸還元活性の株間差異についての検討 (2015)
  • Author(s): 真下千穂，南部隆之
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-12