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Regulation of periodontal disease-related cognitive impairment by controlling microglial phenotype change

Research Project

Project/Area Number 26861555
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionUniversity of Occupational and Environmental Health, Japan (2015-2016)
Kyushu University (2014)

Principal Investigator

Okada Ryo  産業医科大学, 医学部, 講師 (70633105)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsミクログリア / カテプシンB / 歯周病 / ミトコンドリア電子伝達系複合体Ⅰ / 認知症
Outline of Final Research Achievements

Cathepsin B is a lysosomal cysteine protease, and its expression level is elevated at several immune cell types under inflammatory conditions. To clarify the roles of this enzyme on microglial phenotype change, pro-inflammatory M1 phenotype was induced to microglia, and Cathepsin B mRNA expression level was evaluated. This gene expression level was elevated by stimulation with E.coli LPS, but not changed by stimulation with Interferon-γ. These results indicated that this protease expression is regulated by some particular stimulations.
Next, I examined the possibility that periodontal disease-causing bacterium change mammalian brain cell phenotypes by direct interaction. By brain endothelial cell culture with Porphyromonas gingivalis, various inflammatory cytokines were expressed in this mammalian cell. These results suggested that bacteria sensing brain endothelial cells may induce inflammatory conditions and change some brain cell phenotypes at local area in brain.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Cathepsin D deficiency induces oxidative damage in brain pericytes and impairs the blood-brain barrier2015

    • Author(s)
      Yoshinori Hayashi Y, Yuka Koga, Xinwen Zhang, Christoph Peters, Yanagawa Y, Zhou Wu, Takeshi Yokoyama, Hiroshi Nakanishi
    • Journal Title

      Molecular and Cellular Neuroscience

      Volume: 64 Pages: 51-60

    • DOI

      10.1016/j.mcn.2014.12.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] カテプシンH欠損マウスにおける実験的自己免疫性脳脊髄炎の早期発症に関する解析2014

    • Author(s)
      岡田 亮、中西 博
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2014-10-15
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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