Modulation of osteoblast functions by Methylglyoxal
Project/Area Number |
26861558
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Functional basic dentistry
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Research Institution | Showa University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | メチルグリオキサール / 骨芽細胞 / 石灰化 / AGEs / BMP / アルカリホスファターゼ / ナノインデンテーション / 骨芽細胞分化 |
Outline of Final Research Achievements |
Recently attention has been paid to the osteoporosis in diabetic patients. Bone fracture risks increase in type 2 diabetes patients without declining their bone mineral density. It is considered that denaturation of bone matrix by advanced glycation endproducts (AGEs) causes their bone fragility. We examined the effect of methylglyoxal (MG), one of the AGEs, on calcification by osteoblastic MC3T3-E1 cells in vitro. MG caused increase in hardness and decrease in viscoelasticity of calcified nodules formed in the cultures of MC3T3-E1 cells. These results indicate a possibility that MG-induced change in physical properties of the calcified matrix causes the fragility of bones in patients with type 2 diabetes.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor and interleukin-1 but suppresses that by interleukin-17: Importance of proteolytic degradation of osteoprotegerin by lysine gingipain.2014
Author(s)
Tomohito Akiyama, Yoichi Miyamoto, Kentaro Yoshimura, Atsushi Yamada, Masamichi Takami, Tetsuo Suzawa, Marie Hoshino, Takahisa Imamura, Chie Akiyama, Rika Yasuhara, Kenji Mishima, Toshifumi Maruyama, Chikara Kohda, Kazuo Tanaka, Jan Potempa, Hisataka Yasuda, Kazuyoshi Baba, Ryutaro Kamijo.
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Journal Title
J.Biol.Chem.
Volume: 289
Issue: 22
Pages: 1-35
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice.2014
Author(s)
Funato S, Matsunaga A, Oh K, Miyamoto Y, Yoshimura K, Tanaka J, Suzuki D, Uyama R, Suzuki H, Mishima K, Nakamura M, Namiki O, Baba K, Inagaki K, Kamijo R.
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Journal Title
J Negat Results Biomed.
Volume: 13
Issue: 1
Pages: 18-18
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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