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Elucidation of the expression regulatory mechanism of tumor suppressor gene Lefty reprogrammed leukoplakia cells

Research Project

Project/Area Number 26861560
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

Saito Akiko  東京歯科大学, 歯学部, 助手 (90722835)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords疾患iPS細胞 / 癌 / iPS細胞 / Lefty
Outline of Final Research Achievements

Because the appropriate leukoplakia cases could not be obtained, also focused on the basal cell nevus syndrome, a pre-cancerous lesion in and hereditary disease (Gorlin syndrome). We generated four cases of Gorlin patient’ specific iPS cells. In these Gorlin-iPS cells enhanced the ability to proliferate, drug sensitivity in addition serum starvation state had been significantly increased compared to the normal iPS cells. Using our previous report and efficient osteoblast differentiation induction method, after induction of differentiation the Gorlin-iPS cells and normal human iPS cells, was examined the expression of the Hh-related genes using the PCR array. As a result, we found that Gorlin-iPS cells were enhanced the expression of Hh related-genes, Wnt related-genes, BMP and Runx2 compared to the normal iPS cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (13 results)

  • [Journal Article] Promoting effect of 1,25(OH)2 vitamin D3 in osteogenic differentiation from induced pluripotent stem cells to osteocyte like cells2015

    • Author(s)
      Kato H, Ochiai-Shino H, Onodera S, Saito A, Takahiko Shibahara, Azuma T
    • Journal Title

      Open Biology 5(2):140201

      Volume: 5(2) Issue: 2 Pages: 140201-140201

    • DOI

      10.1098/rsob.140201

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Akt Activation is Required for TGF-β1-Induced Osteoblast Differentiation of MC3T3-E1 Pre-Osteoblasts.2015

    • Author(s)
      Suzuki E, Ochiai-Shino H, Aoki H, Onodera S, Saito A, Saito A, Azuma T
    • Journal Title

      Plosons

      Volume: 9(12) Issue: 12 Pages: e112566-e112566

    • DOI

      10.1371/journal.pone.0112566

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.2014

    • Author(s)
      Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T.
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 6 Pages: e99534-e99534

    • DOI

      10.1371/journal.pone.0099534

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 鎖骨頭蓋異形成症患者由来iPS細胞の樹立と機能解析2016

    • Author(s)
      齋藤暁子、大木章生、中村貴、小野寺晶子、篠宏美、長谷川大悟、小崎健次郎、柴原孝彦、末石研二、東俊文
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gorlin 症候群患者由来細胞を用いたiPS 細胞の樹立と骨芽細胞分化の検討2016

    • Author(s)
      篠宏美、長谷川大悟、小野寺晶子、齋藤暁子、大高真奈美、渡邊章、西村健、中西真人、片倉朗、柴原孝彦、東俊文
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gorlin 症候群患者由来iPS 細胞を用いた骨芽細胞分化応答2016

    • Author(s)
      小野寺晶子、大庭伸介、長谷川大悟、齋藤暁子、篠宏美、渡邊章、大高真奈美、西村健、中西真人、片倉朗、鄭雄一、柴原孝彦、東俊文
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者由来細胞を用いた疾患特異的iPS細胞の樹立と機能解析2015

    • Author(s)
      齋藤暁子、大木章生、中村貴、小野寺晶子、篠宏美、長谷川大悟、小崎健次郎、柴原孝彦、末石研二、東俊文
    • Organizer
      第88回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者由来細胞を用いた疾患特異的iPS細胞の樹立と機能解析2015

    • Author(s)
      齋藤暁子、大木章生、中村貴、小野寺晶子、篠宏美、長谷川大悟、小崎健次郎、柴原孝彦、末石研二、東俊文
    • Organizer
      第24回 硬組織再生生物学会学術大会・総会
    • Place of Presentation
      大阪歯科大学
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 鎖骨頭蓋異形成症患者由来細胞を用いた疾患特異的iPS細胞の樹立と機能解析2015

    • Author(s)
      齋藤暁子、中村貴、小野寺晶子、篠宏美、大木章生、長谷川大悟、渡邊章、小崎健次郎、柴原孝彦、末石研二、東俊文
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gorlin 症候群患者由来細胞を用いたヒト人工多能性幹細胞(iPS 細胞)の樹立および骨芽細胞分化2015

    • Author(s)
      小野寺晶子、長谷川大悟、齋藤暁子、大高真奈美、渡邊章、西村健、片倉朗、柴原孝彦、中西真人、東俊文
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 基底細胞癌発生機序解明を目指したGorlin症候群患者由来ヒト人工幹細胞(iPS細胞)の樹立2015

    • Author(s)
      長谷川大悟、小野寺晶子、齋藤暁子、大高真奈美、渡邊章、西村健、東俊文、片倉朗、中西真人、柴原孝彦
    • Organizer
      第24回日本有病者医療学会総会・学術大会
    • Place of Presentation
      旭川市大雪クリスタルホール
    • Year and Date
      2015-03-20 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 鎖骨頭蓋骨異形成症患者由来細胞を用いた疾患特異的iPS細胞の樹立2015

    • Author(s)
      齋藤暁子、小野寺晶子、大木章生、長谷川大悟、大高真奈美、中西真人、西村健、渡邊章、柴原孝彦、末石研二、東俊文
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 基底細胞母斑症候群(Gorlin症候群)患者由来細胞を用いた疾患特異的ヒト人工多能性幹細胞(iPS細胞)の樹立2015

    • Author(s)
      小野寺晶子、長谷川大悟、齋藤暁子、大高真奈美、渡邊章、西村健、柴原孝彦、片倉朗、中西真人、東俊文
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Akt is a Key Modulator in TGF-β1-Induced Osteoblast Differentiation2014

    • Author(s)
      Suzuki E, Ochiai-Shino H, Aoki H, Onodera S, Saito A, Azuma T, Saito A
    • Organizer
      The 62nd Annual, Meeting of Japanese Association for Dental Research, Dec. 2014, Osaka
    • Place of Presentation
      KKR Hotel Osaka
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] iPS細胞から誘導した骨芽細胞を用いた創薬評価-活性型ビタミンD3の効果判定-2014

    • Author(s)
      加藤宏、篠宏美、小野寺晶子、齋藤暁子、林宰央、長谷川大悟、柴原孝彦、東俊文
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report
  • [Presentation] iPS細胞から骨前駆細胞分化誘導および単離の新規方法開発と骨細胞様細胞への分化誘導2014

    • Author(s)
      東俊文、齋藤暁子、小野寺晶子、加藤宏、篠宏美
    • Organizer
      第32回日本骨代謝学会学術集会、2014年7月、大阪市
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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