| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The purpose of this study was to elucidate role of G9a and ESET, both histone methyltransferases, during osteoblastic differentiation. Especially we focused on how they regulate the function of Runx2, which is a critical transcription factor for osteoblastic differentiation. We found that G9a and ESET enhanced transcriptional activity of Runx2. In G9a-knockout osteoblasts, we observed decreased expression of osteocalcin mRNA, while expression of Runx2 was not affected. Moreover, we found that the C-terminal region of G9a was necessary for binding with Runx2 and enhancement its transcription activation. These results suggest that G9a is required for proper function of Runx2 during osteoblastic differentiation.
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