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Modulation of osteoblastic differentiation by G9a and ESET

Research Project

Project/Area Number 26861562
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionTsurumi University

Principal Investigator

Ideno Hisashi  鶴見大学, 歯学部, 学部助手 (40435699)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsエピジェネティクス / ヒストンメチル化 / 骨芽細胞 / 骨芽細胞分化 / 転写制御 / 転写活性化
Outline of Final Research Achievements

The purpose of this study was to elucidate role of G9a and ESET, both histone methyltransferases, during osteoblastic differentiation. Especially we focused on how they regulate the function of Runx2, which is a critical transcription factor for osteoblastic differentiation. We found that G9a and ESET enhanced transcriptional activity of Runx2. In G9a-knockout osteoblasts, we observed decreased expression of osteocalcin mRNA, while expression of Runx2 was not affected. Moreover, we found that the C-terminal region of G9a was necessary for binding with Runx2 and enhancement its transcription activation. These results suggest that G9a is required for proper function of Runx2 during osteoblastic differentiation.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Essential roles of G9a in cell proliferation and differentiation during tooth development.2017

    • Author(s)
      Kamiunten T, Ideno H, Shimada A, Arai Y, Terashima T, Tomooka Y, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A
    • Journal Title

      Experimental Cell Research.

      Volume: 印刷中 Issue: 2 Pages: 170-180

    • DOI

      10.1016/j.yexcr.2017.05.013

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cationized gelatin hydrogels mixed with plasmid DNA induce stronger and more sustained gene expression than atelocollagen at calvarial bone defects in vivo.2016

    • Author(s)
      Komatsu K, Shibata T, Shimada A, Ideno H, Nakashima K, Tabata Y, Nifuji A.
    • Journal Title

      J Biomater Sci Polym Ed.

      Volume: 27 Issue: 5 Pages: 419-430

    • DOI

      10.1080/09205063.2016.1139486

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bardet-Biedl Syndrome 3 regulates development of cranial base midline structures.2016

    • Author(s)
      Kawasaki M, Izu Y, Hayata T, Ideno H, Nifuji A, Sheffield VC, Ezura Y, Noda M.
    • Journal Title

      Bone

      Volume: 89 Pages: 419-430

    • DOI

      10.1016/j.bone.2016.02.017

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Coordinated expression of H3K9 histone methyltransferases during tooth development in mice2015

    • Author(s)
      Kamiunten T, Ideno H, Shimada A, Nakamura Y, Kimura H, Nakashima K, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: 143 Issue: 3 Pages: 259-266

    • DOI

      10.1007/s00418-014-1284-0

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro2015

    • Author(s)
      Wada S, Ideno H, Shimada A, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: in press Issue: 1 Pages: 13-20

    • DOI

      10.1007/s00418-015-1318-2

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Roles of the histone methyltransferase G9a in the development and differentiation of mesenchymal tissues2015

    • Author(s)
      Hisashi Ideno, Kazuhisa Nakashima, Akira Nifuji
    • Journal Title

      The Journal of Physical Fitness and Sports Medicine

      Volume: 4 Issue: 5 Pages: 357-362

    • DOI

      10.7600/jpfsm.4.357

    • NAID

      130005113195

    • ISSN
      2186-8123, 2186-8131
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ヒストンメチル化酵素G9aはマウス歯胚の増殖と分化を制御する2017

    • Author(s)
      出野 尚、上運天太一、島田明美、寺島達夫、中島和久、友岡康弘、中村芳樹、木村 宏、立花 誠、二藤 彰
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] H3K9MTase G9a regulates tooth development in mice2017

    • Author(s)
      Ideno H., Kamiunten T., Shimada A., Terashima T., Nakashima K., Tomooka Y., Nakamura Y., Kimura H., Tachibana M., Nifuji A
    • Organizer
      第39回米国骨代謝学会(ASBMR)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Histone methyltransferase G9a is essential for osteoblastic differentiation and skull bone formation during development2017

    • Author(s)
      Ideno H., Komatsu K., Shimada A., Arai Y., Nakashima K., Tachibana M., Kimura H., Nifuji A)
    • Organizer
      第57回米国細胞生物学会議(ASCB)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒストンメチル化酵素 G9aの骨芽細胞分化における機能2016

    • Author(s)
      出野尚、小松浩一郎、島田明美、新井嘉則、中島和久、荒木良子、安倍真澄、立花誠、木村宏、二藤彰
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Essential roles of H3K9MTase G9a during tooth development2016

    • Author(s)
      出野尚、上運天太一、島田明美、中村芳樹、中島和久、木村宏、立花誠、二藤彰
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2016-07-21
    • Related Report
      2016 Research-status Report
  • [Presentation] Histone methyltransferase G9a is essential for osteoblastic differentiation and proliferation in vitro, and skull bone formation in vivo2015

    • Author(s)
      Hisashi Ideno, Koichiro Komatsu, Akemi Shimada, Taichi Kamiunten, Yoshinori Arai, Kazuhisa Nakashima, Ryoko Araki, Yoshiki Nakamura, Masumi Abe, Makoto Tachibana, Hiroshi Kimura, Akira Nifuji
    • Organizer
      2015 The American Society for Cell Biology Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Deletion of G9a, histone methyltransferase, causes impaired bone formation2015

    • Author(s)
      Hisashi Ideno, Koichiro Komatsu, Akemi Shimada, Taichi Kamiunten, Yoshinori Arai, Kazuhisa Nakashima, Ryoko Araki, Yoshiki Nakamura, Masumi Abe, Makoto Tachibana, Hiroshi Kimura, Akira Nifuji
    • Organizer
      2015 Australia and New Zealand Bone & Mineral Society
    • Place of Presentation
      Hotel Grand Chancellor Hobart, Hobart, TAS, Australia
    • Year and Date
      2015-11-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒストンメチル化酵素G9a欠損マウスは骨形成阻害を示す2015

    • Author(s)
      出野 尚, 小松浩一郎, 島田明美, 上運天太一, 新井嘉則, 中島和久, 荒木良子, 中村芳樹, 安倍真澄, 立花 誠, 木村 宏, 二藤 彰
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル (東京都新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9aによる間葉系細胞の増殖と分化の調節2014

    • Author(s)
      出野 尚、島田明美、上運天太一、和田悟史、小松浩一郎、立花 誠、荒木良子、中村芳樹、安倍真澄、中島和久、二藤 彰
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場・福岡県・福岡市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] The functions of histone methyltransferase G9a during growth and differentiation of mesenchymal cells2014

    • Author(s)
      出野 尚、島田明美、上運天太一、和田悟史、小松浩一郎、荒木良子、中村芳樹、安倍真澄、中島和久、二藤 彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場・大阪府・大阪市
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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