Development of a new dentin regenerative therapy using adipose tissue-derived mesenchymal stem cells

• Project/Area Number: 26861564
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: Mizuno Mitsumasa 愛知学院大学, 歯学部, 助教 (20609812)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生歯科 / 脂肪由来間葉幹細胞 / リコンビナントBMP2 / 骨再生 / 再生医療 / 間葉幹細胞 / ADSC / rhBMP2

Outline of Final Research Achievements

We developed the regenerative therapy of a dention using the rat adipose-derived stem cells (rADSC) which they gathered using magnetic beads from a rat subcutaneous fat tissue in even small facilities in this study. The rADSC had bone differentiation ability,and suggested the possibility of the reproduction treatment of the large bone defect using the three-dimensional molding titanium mesh. In addition, we built the system that could acquire recombinant BMP2 using pCold vector in large quantities and used it for an experiment of afterward. The rADSC had transplanted to the loss part of the rat molar, it was able to confirm the hard tissue reformation 30 days later. The part showed a physiological reaction and was able to confirm a sign of the function recovery.