| Project/Area Number |
26861569
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KAIDA Atsushi 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (40632097)
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| Co-Investigator(Renkei-kenkyūsha) |
MIURA Masahiko 東京医科歯科大学, 医歯学総合研究科, 教授 (10272600)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 放射線治療 / 固形腫瘍 / 細胞動態 / Fucci / 腫瘍内微小環境 / G2アレスト / 細胞周期 / 蛍光イメージング / ライブセルイメージング |
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| Outline of Final Research Achievements |
In this study, we analyzed intratumoral cell cycle kinetics after X-irradiation of tumor xenografts expressing the fluorescent ubiquitination-based cell cycle indicator (Fucci), a novel system to visualize cell cycle kinetics in vivo. Cell cycle kinetics after X-irradiation was examined by using tumor sections and in vivo real-time imaging system in tumor xenografts. We found that G2 arrest was remarkably prolonged, up to 5 days after 10-Gy irradiation, in contrast to monolayer cultures where G2 arrest returned within 24 h. Cells isolated from tumors 5 days after irradiation exhibited a higher surviving fraction than those isolated immediately or one day after irradiation. In this study, we clearly demonstrated unusual post-irradiation cell cycle kinetics in tumor xenografts. Our findings imply that prolonged G2 arrest occurring in tumor microenvironments following irradiation may function as a radioresistance mechanism.
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