BNCT controls the life and death mechanisms of cancer cells.

• Project/Area Number: 26861571
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nara Medical University (2015-2017); Kyoto University (2014)
• Principal Investigator: Nakagawa Yosuke 奈良県立医科大学, 医学部, 助教 (00714875)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: BNCT / BPA / 5-FU / ICP / 細胞周期 / ホウ素中性子捕捉療法 / 化学療法 / CDDP / 移植系動物実験 / 放射線生物学 / 口腔がん

Outline of Final Research Achievements

With neutron radiation after using boron pharmaceuticals（BPA) or X-ray, we collected foundational data, such as the survival rate curve. The relative biological effectiveness (RBE) from the survival rate curve to D10 was from approximately 2.5 to 3.0. Additionally, when we analyzed fluctuations in the cell cycles after irradiation, when isodose 2Gy irradiation was performed, in the neutron radiation group, it was recognized that the cell cycle stopped in the G2/M.
Next, with the transplantation-based animal experiments, an attempt was made to elucidate what cause existing BPA to accumulate in the tumor, and factors to prevent such accumulation. No significant difference was noted in the normal tissue of either group, but in the group to which 5-FU was administered to cancer tissue and 3 days later BPA was administered, a significant decrease in the absorption of boron was recognized.

Research Products

• [Journal Article] Heat Meets DNA: DNA Damage and Repair. (2018)
  • Author(s): Nakagawa Y, Kajihara A, Kirita T, Mori E.
  • Journal Title: Thermal Medicine Volume: 印刷中
  • NAID: 130007437803
  • Peer Reviewed
• [Journal Article] BRCA2 protects mammalian cells from heat shock (2018)
  • Author(s): Yosuke Nakagawa, Atsuhisa Kajihara, Akihisa Takahashi, Akiho S. Murata, Masaya Matsubayashi, Soichiro S. Ito, Ichiro Ota, Takahiko Nakagawa, Masatoshi Hasegawa, Tadaaki Kirita, Takeo Ohnishi, Eiichiro Mori
  • Journal Title: Int J Hyperthermia Volume: 印刷中 Issue: 6 Pages: 1-7
  • DOI: 10.1080/02656736.2017.1370558
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of γH2AX foci in mouse normal brain and brain tumor after boron neutron capture therapy. (2016)
  • Author(s): Kondo N, Michiue H, Sakurai Y, Tanaka H, Nakagawa Y, Watanabe T, Narabayashi M, Kinashi Y, Suzuki M, Masunaga S, Ono K.
  • Journal Title: Rep Pract Oncol Radiother. Volume: 21(2) Issue: 2 Pages: 108-12
  • DOI: 10.1016/j.rpor.2014.10.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparison of the pharmacokinetics between L-BPA and L-FBPA using the same administration dose and protocol: a validation study for the theranostic approach using [18F]-L-FBPA positron emission tomography in boron neutron capture therapy (2016)
  • Author(s): Tsubasa Watanabe, Yoshihide Hattori, Youichiro Ohta, Miki Ishimura, Yosuke Nakagawa, Yu Sanada, Hiroki Tanaka, Satoshi Fukutani, Shin-ichiro Masunaga, Masahiro Hiraoka, Koji Ono, Minoru Suzuki, Mitsunori Kirihata
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 859-868
  • DOI: 10.1186/s12885-016-2913-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Homologous recombination preferentially repairs heat-induced DNA double-strand breaks in mammalian cells (2016)
  • Author(s): Akihisa Takahashi, Eiichiro Mori, Yosuke Nakagawa, Atsuhisa Kajihara, Tadaaki Kirita, Douglas L. Pittman, Masatoshi Hasegawa, Takeo Ohnishi
  • Journal Title: Int J Hyperthermia Volume: 13 Issue: 3 Pages: 1-7
  • DOI: 10.1080/02656736.2016.1252989
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DNA damage induced by boron neutron capture therapy is partially repaired by DNA ligase IV (2016)
  • Author(s): Kondo N, Sakurai Y, Hirota Y, Tanaka H, Watanabe T, Nakagawa Y, Narabayashi M, Kinashi Y, Miyatake S, Hasegawa M, Suzuki M, Masunaga S, Ohnishi T, Ono K.
  • Journal Title: Radiat Environ Biophys Volume: 55(1) Issue: 1 Pages: 89-94
  • DOI: 10.1007/s00411-015-0625-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localized radiation necrosis model in mouse brain using proton ion beams. (2015)
  • Author(s): Kondo N, Sakurai Y, Takata T, Takai N, Nakagawa Y, Tanaka H, Watanabe T, Kume K, Toho T, Miyatake S, Suzuki M, Masunaga S, Ono K.
  • Journal Title: Applied Radiation and Isotopes Volume: 106 Pages: 242-246
  • DOI: 10.1016/j.apradiso.2015.07.032
  • Peer Reviewed / Open Access
• [Journal Article] The BRCA2 gene is a potential molecular target during 5-fluorouracil therapy in human oral cancer cells. (2014)
  • Author(s): Nakagawa Y, Kajihara A, Takahashi A, Kondo N, Mori E, Kirita T, Ohnishi T.
  • Journal Title: Oncology Reports. Volume: 31 Pages: 2001-2006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Significance of administering bevacizumab combined with boron neutron capture therapy in terms of local tumor response and lung metastasis. (2014)
  • Author(s): Masunaga S, Sakurai Y, Tano K, Tanaka H, Suzuki M, Kondo N, Narabayashi M, Watanabe T, Nakagawa Y, Maruhashi A and Ono K.
  • Journal Title: Experimental and Therapeutic Medicine. Volume: ８（１） Pages: 291-301
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The dependency of compound biological effectiveness factors on the type and the concentration of administered neutron capture agents in boron neutron capture therapy. (2014)
  • Author(s): Masunaga S, Sakurai Y, Tanaka H, Tano K, Suzuki M, Kondo N, Narabayashi M, Nakagawa Y, Watanabe T, Maruhashi A and Ono K.
  • Journal Title: Springer Plus. Volume: １２８ Pages: 1-11
  • NAID: 120005439170
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation of thermal neutron irradiation field using a cyclotron-based neutron source for alpha autoradiography. (2014)
  • Author(s): Tanaka H, Sakurai Y, Suzuki M, Masunaga S, Mitsumoto T, Kinashi Y, Kondo N, Narabayashi M, Nakagawa Y, Watanabe T, Fujimoto N, Maruhashi A, Ono K.
  • Journal Title: Applied Radiationand Isotopes. Volume: ８８ Pages: 153-156
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 口腔がん細胞におけるATR 阻害による-FU 増感効果の検討 (2018)
  • Author(s): 仲川洋介、伊藤宗一朗、桐田忠昭
  • Organizer: 第36 回日本口腔腫瘍学会学術大会
• [Presentation] 口腔上皮内腫瘍（OIN）の癌化における腫瘍微小環境とPD-1/PD-L1免疫 (2017)
  • Author(s): 柳生 貴裕、青木 久美子、山川 延宏、上田 順宏、上山 善弘、仲川 洋介、今田 光彦、桐田 忠昭
  • Organizer: 第35回 日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2017-01-26
• [Presentation] ATR inhibition enhances 5-fluorouracil sensitivity in human oral cancer cells. (2017)
  • Author(s): Ito S, Nakagawa Y, Kirita T.
  • Organizer: 6th World Oral Cancer Congress of the International Academy of Oral Oncology (IAOO)
• [Presentation] 経過観察中に舌癌を生じたシェーグレン症候群患者の1例 (2017)
  • Author(s): 仲川洋介、山本一彦、松末友美子、栗原都、桐田忠昭
  • Organizer: 第10回奈良県シェーグレン研究会
• [Presentation] 若年男性の口蓋に発生した多形腺腫由来筋上皮がんの１例 (2016)
  • Author(s): 仲川洋介、下村弘幸、下村忠弘、柳生貴裕、玉置盛浩、桐田忠昭
  • Organizer: 第61回 日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2016-11-25
• [Presentation] ホウ素中性子捕捉療法における均一な熱中性子場形成に関する研究 (2015)
  • Author(s): 藤本 望、田中 浩基、櫻井 良憲、高田 卓志、近藤 夏子、楢林 正流、仲川 洋介、渡邉 翼、木梨 友子、増永 慎一郎、丸橋 晃、小野 公二、鈴木 実
  • Organizer: 第12回日本中性子捕捉療法学会学術大会
  • Place of Presentation: 神戸学院大学
  • Year and Date: 2015-09-04
• [Presentation] Radiation and Inflammation (2015)
  • Author(s): 仲川洋介
  • Organizer: 第36回 夏季放射線腫瘍学セミナー
  • Place of Presentation: 紀伊見荘(橋本市）
  • Year and Date: 2015-08-01
• [Presentation] 京都大学原子炉実験所におけるBNCTの臨床研究 (2015)
  • Author(s): 仲川洋介
  • Organizer: 岡山大学歯学部同窓会奈良県支部学術集会
  • Place of Presentation: 奈良県歯科医師会館
  • Year and Date: 2015-06-28
  • Invited
• [Presentation] 口腔がん細胞における浸透圧ストレスを利用した放射線増感効果の検討 (2014)
  • Author(s): 仲川洋介、梶原淳久、桐田忠昭
  • Organizer: 第59回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉県幕張メッセ
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] 京都大学原子炉実験所症例報告(頭頸部) (2014)
  • Author(s): 仲川洋介
  • Organizer: 平成26年度京都大学原子炉実験所専門研究会
  • Place of Presentation: 京都大学原子炉実験所
  • Year and Date: 2014-09-29
• [Remarks] 奈良県立口腔外科学講座
  • URL: http://www.naramed-u.ac.jp/~oral/
• [Remarks] 京都大学教育研究活動データベース
  • URL: http://kyouindb.iimc.kyoto-u.ac.jp/e/jZ1yN